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Cell Discov January 1, 2017; 3 17046.

Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse.

Chen Y , Xu H , Lin G .

The capacity of digit tip regeneration observed both in rodents and humans establishes a foundation for promoting robust regeneration in mammals. However, stimulating regeneration at more proximal levels, such as the middle phalanges (P2) of the adult mouse, remains challenging. Having shown the effectiveness of transplantation of limb progenitor cells in stimulating limb regeneration in Xenopus, we are now applying the cell transplantation approach to the adult mouse. Here we report that both embryonic and induced pluripotent stem cell (iPSC)-derived limb progenitor-like cells can promote adult mouse P2 regeneration. We have established a simple and efficient protocol for deriving limb progenitor-like cells from mouse iPSCs. iPSCs are cultured as three-dimensional fibrin bodies, followed by treatment with combinations of Fgf8, CHIR99021, Purmorphamine and SB43542 during differentiation. These iPSC-derived limb progenitor-like cells resemble embryonic limb mesenchyme cells in their expression of limb-related genes. After transplantation, the limb progenitor-like cells can promote adult mouse P2 regeneration, as embryonic limb bud cells do. Our results provide a basis for further developing progenitor cell-based approaches for improving regeneration in the adult mouse limbs.

PubMed ID: 29263795
PMC ID: PMC5735367
Article link: Cell Discov
Grant support: [+]

Species referenced: Xenopus
Genes referenced: acta2 fbrs fgf10 fgf8 gli3 isl1 kdr lif pitx1 pou5f3.1 tbx4 tbx5

Article Images: [+] show captions
References [+] :
Agrawal, Recruitment of progenitor cells by an extracellular matrix cryptic peptide in a mouse model of digit amputation. 2012, Pubmed