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XB-ART-54657
J Cell Biol January 1, 2018; 217 (5): 1633-1641.
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CLAMP/Spef1 regulates planar cell polarity signaling and asymmetric microtubule accumulation in the Xenopus ciliated epithelia.

Kim SK , Zhang S , Werner ME , Brotslaw EJ , Mitchell JW , Altabbaa MM , Mitchell BJ .


Abstract
Most epithelial cells polarize along the axis of the tissue, a feature known as planar cell polarity (PCP). The initiation of PCP requires cell-cell signaling via the noncanonical Wnt/PCP pathway. Additionally, changes in the cytoskeleton both facilitate and reflect this polarity. We have identified CLAMP/Spef1 as a novel regulator of PCP signaling. In addition to decorating microtubules (MTs) and the ciliary rootlet, a pool of CLAMP localizes at the apical cell cortex. Depletion of CLAMP leads to the loss of PCP protein asymmetry, defects in cilia polarity, and defects in the angle of cell division. Additionally, depletion of CLAMP leads to a loss of the atypical cadherin-like molecule Celrs2, suggesting that CLAMP facilitates the stabilization of junctional interactions responsible for proper PCP protein localization. Depletion of CLAMP also affects the polarized organization of MTs. We hypothesize that CLAMP facilitates the establishment of cell polarity and promotes the asymmetric accumulation of MTs downstream of the establishment of proper PCP.

PubMed ID: 29514918
PMC ID: PMC5940297
Article link: J Cell Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: dvl1 mcc mcidas mid1ip1 prok2 spef1 tgfbi vangl2
GO keywords: planar cell polarity pathway involved in axis elongation [+]


Article Images: [+] show captions
References [+] :
Boutin, A dual role for planar cell polarity genes in ciliated cells. 2014, Pubmed