Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Cell Rep January 1, 2018; 24 (2): 304-311.
Show Gene links Show Anatomy links

Chromatin Accessibility Impacts Transcriptional Reprogramming in Oocytes.

Miyamoto K , Nguyen KT , Allen GE , Jullien J , Kumar D , Otani T , Bradshaw CR , Livesey FJ , Kellis M , Gurdon JB .

Oocytes have a remarkable ability to reactivate silenced genes in somatic cells. However, it is not clear how the chromatin architecture of somatic cells affects this transcriptional reprogramming. Here, we investigated the relationship between the chromatin opening and transcriptional activation. We reveal changes in chromatin accessibility and their relevance to transcriptional reprogramming after transplantation of somatic nuclei into Xenopus oocytes. Genes that are silenced, but have pre-existing open transcription start sites in donor cells, are prone to be activated after nuclear transfer, suggesting that the chromatin signature of somatic nuclei influences transcriptional reprogramming. There are also activated genes associated with new open chromatin sites, and transcription factors in oocytes play an important role in transcriptional reprogramming from such genes. Finally, we show that genes resistant to reprogramming are associated with closed chromatin configurations. We conclude that chromatin accessibility is a central factor for successful transcriptional reprogramming in oocytes.

PubMed ID: 29996092
PMC ID: PMC6057489
Article link: Cell Rep
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: ap1s3 cfp fnbp1l h2bc21 hoxc8 pou5f3.1 rab40b rara
GO keywords: chromatin reprogramming in the zygote

Article Images: [+] show captions
References [+] :
Adelman, Promoter-proximal pausing of RNA polymerase II: emerging roles in metazoans. 2012, Pubmed