XB-ART-55238
Methods Mol Biol
2018 Jan 01;1865:105-117. doi: 10.1007/978-1-4939-8784-9_8.
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How to Generate Non-Mosaic CRISPR/Cas9 Mediated Knock-In and Mutations in F0 Xenopus Through the Host-Transfer Technique.
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We have taken advantage of the well-established oocyte host transfer technique to optimize a method for CRISPR editing of Xenopus that provides an efficient non-mosaic targeted insertion of small DNA fragment through homology-directed repair mechanism.
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