Dong C et al. (2018), Expression of trpv channels during Xenopus laev...

XB-ART-55388

Gene Expr Patterns 2018 Dec 01;30:64-70. doi: 10.1016/j.gep.2018.10.001.

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Expression of trpv channels during Xenopus laevis embryogenesis.

Dong C , Paudel S , Amoh NY , Saha MS .

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Transient receptor potential (TRP) cation channel genes code for an extensive family of conserved proteins responsible for a variety of physiological processes, including sensory perception, ion homeostasis, and chemical signal transduction. The TRP superfamily consists of seven subgroups, one of which is the transient receptor potential vanilloid (trpv) channel family. While trpv channels are relatively well studied in adult vertebrate organisms given their role in functions such as nociception, thermoregulation, and osmotic regulation in mature tissues and organ systems, relatively little is known regarding their function during embryonic development. Although there are some reports of the expression of specific trpv channels at particular stages in various organisms, there is currently no comprehensive analysis of trpv channels during embryogenesis. Here, performing in situ hybridization, we examined the spatiotemporal expression of trpv channel mRNA during early Xenopus laevis embryogenesis. Trpv channels exhibited unique patterns of embryonic expression at distinct locations including the trigeminal ganglia, spinal cord, cement gland, otic vesicle, optic vesicle, nasal placode, notochord, tailbud, proctodeum, branchial arches, epithelium, somite and the animal pole during early development. We have also observed the colocalization of trpv channels at the animal pole (trpv 2/4), trigeminal ganglia (trpv 1/2), and epithelium (trpv 5/6). These localization patterns suggest that trpv channels may play diverse roles during early embryonic development.

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Species referenced: Xenopus laevis
Genes referenced: trpv1 trpv2 trpv4 trpv5 trpv6
GO keywords: embryo development [+]

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	Fig. 1. Whole mount (A–D) and histological (A′-D‴) expression of trpv1 transcripts in developing X. laevis embryos. (A–B) dorsal view, (C–D) lateral view, anterior to the right for all whole mount embryos; dorsal to the top for all histology images. (A, A′, A′) stage 20 (late neurula stage); (B, B′, B′) stage 25 (early tailbud stage); (C, C′, C′) stage 30 (late tailbud stage); (D, D′, D′, D‴) stage 35 (swimming tadpole stage). Arrows indicate regions of gene expression (nt, nural tube; sc, spinal cord; tg, trigeminal ganglia). Dashed lines represent positions of corresponding sections. Scale bars = 250 μm.
	Fig. 2. Whole mount (A–F) and histological (A′-F⁗) expression of trpv2 transcripts in X. laevis embryos. Lateral view for all whole mount embryos, animal pole to the top (A–C), anterior to the right (D–F); dorsal to the top for all histology images. (A, A′) unfertilized egg; (B, B′) stage 5 (16-cell stage); (C, C′) stage 8 (mid-blastula stage); (D, D′, D′) stage 25 (early tailbud stage); (E, E′, E′, E‴, E⁗;) stage 30 (late tailbud stage); (F, F′, F′, F‴, F⁗) stage 35 (swimming tadpole stage). Arrows indicate regions of gene expression (a, animal pole; cg, cement gland; nt, neural tube; op, optic vesicle; ot, otic vesicle; tg, trigeminal ganglia; sc, spinal cord). Dashed lines represent positions of corresponding sections. Scale bars = 250 μm.
	Fig. 3. Whole mount (A–G) and histological (A′-G′) expression of trpv4 mRNA expression in X. laevis embryos. Lateral view for all whole mount embryos, animal pole to the top (A–D), anterior to the right (E–G); dorsal to the top for all histology images. (A, A′) unfertilized egg; (B, B′) stage 5 (16-cell stage); (C, C′) stage 8 (mid-blastula stage); (D, C′) stage 10 (early gastrula stage); (E, E′) stage 20 (late neurula stage); (F, F′, F′) stage 30 (late tailbud stage); (G, G′, G′) stage 35 (swimming tadpole stage). Arrows indicate regions of gene expression (a, animal pole; nc, notochord; np, nasal placode; tb, tailbud). Dashed lines represent positions of corresponding sections. Scale bars = 250 μm.
	Fig. 4. Whole mount (A–E) and histological (A′-E′) expression of trpv5 transcripts in developing X. laevis embryos. (A) dorsal view, (B–E) lateral view, anterior to the right for all whole mount embryos; dorsal to the top for all histology images. (A, A′) stage 15 (mid-neurula stage); (B, B′, B′) stage 20 (late neurula stage); (C, C′, C′) stage 25 (early tailbud stage); (D, D′, D′) stage 30 (late tailbud stage); (E, E′) stage 35 (swimming tadpole stage). Arrows indicate regions of gene expression (ba, branchial arches; ep, epidermis; pd, proctodeum). Dashed lines represent positions of corresponding sections. Scale bars = 250 μm.
	Fig. 5. Whole mount (A–E) and histological (A′-E′) expression of trpv6 transcripts in X. laevis embryos. Lateral view for all whole mount embryos, anterior to the right; dorsal to the top for all histology images. (A, A′) stage 15 (mid-neurula stage); (B, B′) stage 20 (late neurula stage); (C, C′) stage 25 (early tailbud stage); (D, D′) stage 30 (late tailbud stage); (E, E′) stage 35 (swimming tadpole stage). Arrows indicate regions of gene expression (ep, epidermis). Dashed lines represent positions of corresponding sections. Scale bars = 250 μm.

References [+] :

Antin, Gallus expression in situ hybridization analysis: a chicken embryo gene expression database. 2007, Pubmed