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Plant Physiol
2019 Feb 01;1792:656-670. doi: 10.1104/pp.18.01097.
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OsPHT1;3 Mediates Uptake, Translocation, and Remobilization of Phosphate under Extremely Low Phosphate Regimes.
Chang MX
,
Gu M
,
Xia YW
,
Dai XL
,
Dai CR
,
Zhang J
,
Wang SC
,
Qu HY
,
Yamaji N
,
Feng Ma J
,
Xu GH
.
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Plant roots rely on inorganic orthophosphate (Pi) transporters to acquire soluble Pi from soil solutions that exists at micromolar levels in natural ecosystems. Here, we functionally characterized a rice (Oryza sativa) Pi transporter, Os Phosphate Transporter-1;3 (OsPHT1;3), that mediates Pi uptake, translocation, and remobilization. OsPHT1;3 was directly regulated by Os Phosphate Starvation Response-2 and, in response to Pi starvation, showed enhanced expression in young leaf blades and shoot basal regions and even more so in roots and old leaf blades. OsPHT1;3 was able to complement a yeast mutant strain defective in five Pi transporters and mediate Pi influx in Xenopus laevis oocytes. Overexpression of OsPHT1;3 led to increased Pi concentration both in roots and shoots. However, unlike that reported for other known OsPHT1 members that facilitate Pi uptake at relatively higher Pi levels, mutation of OsPHT1;3 impaired Pi uptake and root-to-shoot Pi translocation only when external Pi concentration was below 5 μm Moreover, in basal nodes, the expression of OsPHT1;3 was restricted to the phloem of regular vascular bundles and enlarged vascular bundles. An isotope labeling experiment with 32P showed that ospht1;3 mutant lines were impaired in remobilization of Pi from source to sink leaves. Furthermore, overexpression and mutation of OsPHT1;3 led to reciprocal alteration in the expression of OsPHT1;2 and several other OsPHT1 genes. Yeast-two-hybrid, bimolecular fluorescence complementation, and coimmunoprecipitation assays all demonstrated a physical interaction between OsPHT1;3 and OsPHT1;2. Taken together, our results indicate that OsPHT1;3 acts as a crucial factor for Pi acquisition, root-to-shoot Pi translocation, and redistribution of phosphorus in plants growing in environments with extremely low Pi levels.
Ai,
Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation.
2009, Pubmed,
Xenbase
Ai,
Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation.
2009,
Pubmed
,
Xenbase
Aung,
pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene.
2006,
Pubmed
Ayadi,
Reducing the genetic redundancy of Arabidopsis PHOSPHATE TRANSPORTER1 transporters to study phosphate uptake and signaling.
2015,
Pubmed
Bustos,
A central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in Arabidopsis.
2010,
Pubmed
Chen,
Conservation and divergence of both phosphate- and mycorrhiza-regulated physiological responses and expression patterns of phosphate transporters in solanaceous species.
2007,
Pubmed
Chen,
The rice CK2 kinase regulates trafficking of phosphate transporters in response to phosphate levels.
2015,
Pubmed
Chiou,
The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface.
2001,
Pubmed
Fontenot,
Increased phosphate transport of Arabidopsis thaliana Pht1;1 by site-directed mutagenesis of tyrosine 312 may be attributed to the disruption of homomeric interactions.
2015,
Pubmed
Glass,
A reevaluation of the role of Arabidopsis NRT1.1 in high-affinity nitrate transport.
2013,
Pubmed
Gu,
Complex Regulation of Plant Phosphate Transporters and the Gap between Molecular Mechanisms and Practical Application: What Is Missing?
2016,
Pubmed
Guo,
Integrative Comparison of the Role of the PHOSPHATE RESPONSE1 Subfamily in Phosphate Signaling and Homeostasis in Rice.
2015,
Pubmed
Hong,
Convergent starvation signals and hormone crosstalk in regulating nutrient mobilization upon germination in cereals.
2012,
Pubmed
Huang,
Identification of downstream components of ubiquitin-conjugating enzyme PHOSPHATE2 by quantitative membrane proteomics in Arabidopsis roots.
2013,
Pubmed
Javot,
A Medicago truncatula phosphate transporter indispensable for the arbuscular mycorrhizal symbiosis.
2007,
Pubmed
Jia,
The phosphate transporter gene OsPht1;8 is involved in phosphate homeostasis in rice.
2011,
Pubmed
,
Xenbase
Kalkhajeh,
Phosphorus saturation and mobilization in two typical Chinese greenhouse vegetable soils.
2017,
Pubmed
Lapis-Gaza,
Arabidopsis PHOSPHATE TRANSPORTER1 genes PHT1;8 and PHT1;9 are involved in root-to-shoot translocation of orthophosphate.
2014,
Pubmed
Li,
Phosphate transporter OsPht1;8 in rice plays an important role in phosphorus redistribution from source to sink organs and allocation between embryo and endosperm of seeds.
2015,
Pubmed
Li,
Generation of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System.
2017,
Pubmed
Lin,
Nitrogen limitation adaptation, a target of microRNA827, mediates degradation of plasma membrane-localized phosphate transporters to maintain phosphate homeostasis in Arabidopsis.
2013,
Pubmed
Liu,
OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice.
2010,
Pubmed
Miao,
Targeted mutagenesis in rice using CRISPR-Cas system.
2013,
Pubmed
Mudge,
Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis.
2002,
Pubmed
Nagarajan,
Arabidopsis Pht1;5 mobilizes phosphate between source and sink organs and influences the interaction between phosphate homeostasis and ethylene signaling.
2011,
Pubmed
Nussaume,
Phosphate Import in Plants: Focus on the PHT1 Transporters.
2011,
Pubmed
Pedersen,
Crystal structure of a eukaryotic phosphate transporter.
2013,
Pubmed
Preuss,
Channel-like characteristics of the low-affinity barley phosphate transporter PHT1;6 when expressed in Xenopus oocytes.
2010,
Pubmed
,
Xenbase
Rae,
Characterization of two phosphate transporters from barley; evidence for diverse function and kinetic properties among members of the Pht1 family.
2003,
Pubmed
Raghothama,
PHOSPHATE ACQUISITION.
1999,
Pubmed
Remy,
The Pht1;9 and Pht1;8 transporters mediate inorganic phosphate acquisition by the Arabidopsis thaliana root during phosphorus starvation.
2012,
Pubmed
Robinson,
Eliminating the purple acid phosphatase AtPAP26 in Arabidopsis thaliana delays leaf senescence and impairs phosphorus remobilization.
2012,
Pubmed
Ruan,
Phosphate starvation induced OsPHR4 mediates Pi-signaling and homeostasis in rice.
2017,
Pubmed
Rubio,
A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae.
2001,
Pubmed
Sasaki,
Overexpression of OsHMA3 enhances Cd tolerance and expression of Zn transporter genes in rice.
2014,
Pubmed
Secco,
Spatio-temporal transcript profiling of rice roots and shoots in response to phosphate starvation and recovery.
2013,
Pubmed
Shin,
Phosphate transport in Arabidopsis: Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low- and high-phosphate environments.
2004,
Pubmed
Sun,
A constitutive expressed phosphate transporter, OsPht1;1, modulates phosphate uptake and translocation in phosphate-replete rice.
2012,
Pubmed
Sun,
Arabidopsis PHL2 and PHR1 Act Redundantly as the Key Components of the Central Regulatory System Controlling Transcriptional Responses to Phosphate Starvation.
2016,
Pubmed
Tong,
A two-component high-affinity nitrate uptake system in barley.
2005,
Pubmed
,
Xenbase
Wang,
Phosphate transporters OsPHT1;9 and OsPHT1;10 are involved in phosphate uptake in rice.
2014,
Pubmed
,
Xenbase
Wang,
Rice SPX-Major Facility Superfamily3, a Vacuolar Phosphate Efflux Transporter, Is Involved in Maintaining Phosphate Homeostasis in Rice.
2015,
Pubmed
,
Xenbase
Wu,
Does OsPHR2, central pi-signaling regulator, regulate some unknown factors crucial for plant growth?
2010,
Pubmed
Wykoff,
Phosphate transport and sensing in Saccharomyces cerevisiae.
2001,
Pubmed
Yamaji,
The node, a hub for mineral nutrient distribution in graminaceous plants.
2014,
Pubmed
Yamaji,
Node-controlled allocation of mineral elements in Poaceae.
2017,
Pubmed
Yamaji,
Reducing phosphorus accumulation in rice grains with an impaired transporter in the node.
2017,
Pubmed
Yamaji,
Spatial distribution and temporal variation of the rice silicon transporter Lsi1.
2007,
Pubmed
Yang,
Nonredundant regulation of rice arbuscular mycorrhizal symbiosis by two members of the phosphate transporter1 gene family.
2012,
Pubmed
Yue,
OsNLA1, a RING-type ubiquitin ligase, maintains phosphate homeostasis in Oryza sativa via degradation of phosphate transporters.
2017,
Pubmed
Zhang,
Involvement of OsPht1;4 in phosphate acquisition and mobilization facilitates embryo development in rice.
2015,
Pubmed
,
Xenbase
Zhou,
OsPHR2 is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants.
2008,
Pubmed
