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XB-ART-5568
Dev Biol March 15, 2003; 255 (2): 290-302.

Difference in the maternal and zygotic contributions of tumorhead on embryogenesis.

Wu CF , Chan AP , Etkin LD .


Abstract
Tumorhead (TH) is a maternally expressed gene in Xenopus laevis, that when overexpressed, increased proliferation of ectodermal derivatives and inhibited neural and epidermal differentiation. However, injection of anti-TH antibodies inhibited cleavage of all blastomeres, not only those contributing to the ectoderm. The injection of TH morpholino antisense oligonucleotide (TH-MO), which inhibits translation of TH mRNA, did not affect early cleavage but inhibited cell division in both the neural field and epidermis. This was accompanied by the inhibition of neural and epidermal markers. TH-MO did not affect the formation and differentiation of mesoderm and endoderm derivatives. Our overexpression and loss-of-function studies demonstrated that TH plays an important role in differentiation of the ectoderm by regulating cell proliferation. They also supported the conclusion that the maternal component of TH may affect the cell cycle in all cells, while the zygotic component has a germ layer-specific effect on the ectoderm.

PubMed ID: 12648491
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus
Genes referenced: trhd
Antibodies: Somite Ab1 Trhd Ab1
Morpholinos: trhd MO1