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XB-ART-55770
EMBO Rep January 1, 2019; 20 (5):
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Structural basis of HEAT-kleisin interactions in the human condensin I subcomplex.

Hara K , Kinoshita K , Migita T , Murakami K , Shimizu K , Takeuchi K , Hirano T , Hashimoto H .


Abstract
Condensin I is a multi-protein complex that plays an essential role in mitotic chromosome assembly and segregation in eukaryotes. It is composed of five subunits: two SMC (SMC2 and SMC4), a kleisin (CAP-H), and two HEAT-repeat (CAP-D2 and CAP-G) subunits. Although balancing acts of the two HEAT-repeat subunits have been demonstrated to enable this complex to support the dynamic assembly of chromosomal axes in vertebrate cells, its underlying mechanisms remain poorly understood. Here, we report the crystal structure of a human condensin I subcomplex comprising hCAP-G and hCAP-H. hCAP-H binds to the concave surfaces of a harp-shaped HEAT-repeat domain of hCAP-G. Physical interaction between hCAP-G and hCAP-H is indeed essential for mitotic chromosome assembly recapitulated in Xenopus egg cell-free extracts. Furthermore, this study reveals that the human CAP-G-H subcomplex has the ability to interact with not only double-stranded DNA, but also single-stranded DNA, suggesting functional divergence of the vertebrate condensin I complex in proper mitotic chromosome assembly.

PubMed ID: 30858338
PMC ID: PMC6501013
Article link: EMBO Rep


Species referenced: Xenopus laevis
Genes referenced: angptl2 pou3f3 smc2 smc4
GO keywords: condensin I complex

Disease Ontology terms: microcephaly

Article Images: [+] show captions
References [+] :
Adams, PHENIX: a comprehensive Python-based system for macromolecular structure solution. 2010, Pubmed