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Nanomaterials (Basel) April 9, 2019; 9 (4):
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Thermal Reduction of Graphene Oxide Mitigates Its In Vivo Genotoxicity Toward Xenopus laevis Tadpoles.

Evariste L , Lagier L , Gonzalez P , Mottier A , Mouchet F , Cadarsi S , Lonchambon P , Daffe G , Chimowa G , Sarrieu C , Ompraret E , Galibert AM , Ghimbeu CM , Pinelli E , Flahaut E , Gauthier L .

The worldwide increase of graphene family materials raises the question of the potential consequences resulting from their release in the environment and future consequences on ecosystem health, especially in the aquatic environment in which they are likely to accumulate. Thus, there is a need to evaluate the biological and ecological risk but also to find innovative solutions leading to the production of safer materials. This work focuses on the evaluation of functional group-safety relationships regarding to graphene oxide (GO) in vivo genotoxic potential toward X. laevis tadpoles. For this purpose, thermal treatments in H₂ atmosphere were applied to produce reduced graphene oxide (rGOs) with different surface group compositions. Analysis performed indicated that GO induced disturbances in erythrocyte cell cycle leading to accumulation of cells in G0/G1 phase. Significant genotoxicity due to oxidative stress was observed in larvae exposed to low GO concentration (0.1 mg.L-¹). Reduction of GO at 200 °C and 1000 °C produced a material that was no longer genotoxic at low concentrations. X-ray photoelectron spectroscopy (XPS) analysis indicated that epoxide groups may constitute a good candidate to explain the genotoxic potential of the most oxidized form of the material. Thermal reduction of GO may constitute an appropriate "safer-by-design" strategy for the development of a safer material for environment.

PubMed ID: 30970633
PMC ID: PMC6523888
Article link: Nanomaterials (Basel)
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: alox5 cat.2 cyp1a1 gpx1 gstp1 lta4h mlh1 ptgs1 rad51 sod1 sod2
GO keywords: response to reactive oxygen species [+]

Phenotypes: Xla Wt + Cyclophosphamide hydrate (Fig. 5) [+]

Article Images: [+] show captions
References [+] :
Araldi, Using the comet and micronucleus assays for genotoxicity studies: A review. 2016, Pubmed