XB-ART-55948Front Physiol January 1, 2019; 10 387.
Xenopus Resources: Transgenic, Inbred and Mutant Animals, Training Opportunities, and Web-Based Support.
Two species of the clawed frog family, Xenopus laevis and X. tropicalis, are widely used as tools to investigate both normal and disease-state biochemistry, genetics, cell biology, and developmental biology. To support both frog specialist and non-specialist scientists needing access to these models for their research, a number of centralized resources exist around the world. These include centers that hold live and frozen stocks of transgenic, inbred and mutant animals and centers that hold molecular resources. This infrastructure is supported by a model organism database. Here, we describe much of this infrastructure and encourage the community to make the best use of it and to guide the resource centers in developing new lines and libraries.
PubMed ID: 31073289
PMC ID: PMC6497014
Article link: Front Physiol
Species referenced: Xenopus Xenopus tropicalis
Genes referenced: runx1
Article Images: [+] show captions
|FIGURE 1. Labeling haematopoietic stem cells in live embryos. Xenopus laevis eggs were microinjected with CRISPR/Cas9 targeting the 3′UTR of runx1 together with a DNA construct containing 400 bp homology arms from either side of the cut site flanking an IRES controlling eGFP expression. Founder embryos were screened for mosaic expression of GFP in the correct regions and 24 grown to adulthood. Their offspring were then screened for germline transmission; two sets of offspring showed strong expression in the vbi (A, arrowed) and vasculature (B, examples arrowed) as expected for runx1 at this stage. The transmission rate was 46 and 52% in the 2 sets. An F2 embryo is shown.|
|FIGURE 2. Genetic relationships and heterozygosity reduction curve in the strains of X. tropicalis. (A) Neighbor joining trees of the strains based on genetic distances (DA) of 60 Simple Sequence Length Polymorphism (SSLP) markers (upper) and p-distance of mitochondrial haplotypes (2,328 bp; lower). Numbers on branches indicate percent bootstrap probability (only indicated > 50%). (B) Plot of expected heterozygosity (HE) against generation number (t). The curved dotted strain indicates theoretical reduction of heterozygosity via single brother-sister matings for every generation, calculated from the following equation: HE = H0(1−12Ne)t.|
References [+] :
Allen, Transgenic Xenopus laevis embryos can be generated using phiC31 integrase. 2006, Pubmed, Xenbase