XB-ART-56361Cell Death Differ January 1, 2020; 27 (4): 1225-1242.
LIN28B increases neural crest cell migration and leads to transformation of trunk sympathoadrenal precursors.
The RNA-binding protein LIN28B regulates developmental timing and determines stem cell identity by suppressing the let-7 family of microRNAs. Postembryonic reactivation of LIN28B impairs cell commitment to differentiation, prompting their transformation. In this study, we assessed the extent to which ectopic lin28b expression modulates the physiological behavior of neural crest cells (NCC) and governs their transformation in the trunk region of developing embryos. We provide evidence that the overexpression of lin28b inhibits sympathoadrenal cell differentiation and accelerates NCC migration in two vertebrate models, Xenopus leavis and Danio rerio. Our results highlight the relevance of ITGA5 and ITGA6 in the LIN28B-dependent regulation of the invasive motility of tumor cells. The results also establish that LIN28B overexpression supports neuroblastoma onset and the metastatic potential of malignant cells through let-7a-dependent and let-7a-independent mechanisms.
PubMed ID: 31601998
PMC ID: PMC7206034
Article link: Cell Death Differ
Species referenced: Xenopus laevis
Genes referenced: itga5 itga6 lin28b slc12a3
References [+] :
Allende, The expression pattern of two zebrafish achaete-scute homolog (ash) genes is altered in the embryonic brain of the cyclops mutant. 1995, Pubmed, Xenbase