Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cold Spring Harb Protoc
2020 Feb 03;20202:098350. doi: 10.1101/pdb.top098350.
Show Gene links
Show Anatomy links
Transcriptomics and Proteomics Methods for Xenopus Embryos and Tissues.
Gilchrist MJ
,
Veenstra GJC
,
Cho KWY
.
???displayArticle.abstract???
The general field of quantitative biology has advanced significantly on the back of recent improvements in both sequencing technology and proteomics methods. The development of high-throughput, short-read sequencing has revolutionized RNA-based expression studies, while improvements in proteomics methods have enabled quantitative studies to attain better resolution. Here we introduce methods to undertake global analyses of gene expression through RNA and protein quantification in Xenopus embryos and tissues.
Amin,
Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT).
2014, Pubmed,
Xenbase
Amin,
Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT).
2014,
Pubmed
,
Xenbase
Blitz,
A catalog of Xenopus tropicalis transcription factors and their regional expression in the early gastrula stage embryo.
2017,
Pubmed
,
Xenbase
Briggs,
The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution.
2018,
Pubmed
,
Xenbase
Butler,
A novel role for sox7 in Xenopus early primordial germ cell development: mining the PGC transcriptome.
2018,
Pubmed
,
Xenbase
Charney,
Foxh1 Occupies cis-Regulatory Modules Prior to Dynamic Transcription Factor Interactions Controlling the Mesendoderm Gene Program.
2017,
Pubmed
,
Xenbase
Collart,
High-resolution analysis of gene activity during the Xenopus mid-blastula transition.
2014,
Pubmed
,
Xenbase
De Domenico,
Molecular asymmetry in the 8-cell stage Xenopus tropicalis embryo described by single blastomere transcript sequencing.
2015,
Pubmed
,
Xenbase
Ding,
Genome-wide analysis of dorsal and ventral transcriptomes of the Xenopus laevis gastrula.
2017,
Pubmed
,
Xenbase
Eng,
Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH.
2019,
Pubmed
Klein,
Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells.
2015,
Pubmed
,
Xenbase
Laver,
Assessing the performance of the Oxford Nanopore Technologies MinION.
2015,
Pubmed
Lindeboom,
Mass Spectrometry-Based Absolute Quantification of Single Xenopus Embryo Proteomes.
2019,
Pubmed
,
Xenbase
Lombard-Banek,
Label-free Quantification of Proteins in Single Embryonic Cells with Neural Fate in the Cleavage-Stage Frog (Xenopus laevis) Embryo using Capillary Electrophoresis Electrospray Ionization High-Resolution Mass Spectrometry (CE-ESI-HRMS).
2016,
Pubmed
,
Xenbase
McCarthy,
Third generation DNA sequencing: pacific biosciences' single molecule real time technology.
2010,
Pubmed
Onjiko,
Single-cell mass spectrometry reveals small molecules that affect cell fates in the 16-cell embryo.
2015,
Pubmed
,
Xenbase
Owens,
Measuring Absolute RNA Copy Numbers at High Temporal Resolution Reveals Transcriptome Kinetics in Development.
2016,
Pubmed
,
Xenbase
Owens,
An RNA-Seq Protocol for Differential Expression Analysis.
2019,
Pubmed
,
Xenbase
Palii,
Single-Cell Proteomics Reveal that Quantitative Changes in Co-expressed Lineage-Specific Transcription Factors Determine Cell Fate.
2019,
Pubmed
Paraiso,
Endodermal Maternal Transcription Factors Establish Super-Enhancers during Zygotic Genome Activation.
2019,
Pubmed
,
Xenbase
Peshkin,
On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development.
2015,
Pubmed
,
Xenbase
Presler,
Proteomics of phosphorylation and protein dynamics during fertilization and meiotic exit in the Xenopus egg.
2017,
Pubmed
,
Xenbase
Smits,
Global absolute quantification reveals tight regulation of protein expression in single Xenopus eggs.
2014,
Pubmed
,
Xenbase
Wasson,
INTACT Proteomics in Xenopus.
2019,
Pubmed
,
Xenbase
Wühr,
Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database.
2014,
Pubmed
,
Xenbase
