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Cell Rep January 1, 2018; 24 (13): 3477-3487.e6.

Nut Directs p300-Dependent, Genome-Wide H4 Hyperacetylation in Male Germ Cells.

Shiota H , Barral S , Buchou T , Tan M , Couté Y , Charbonnier G , Reynoird N , Boussouar F , Gérard M , Zhu M , Bargier L , Puthier D , Chuffart F , Bourova-Flin E , Picaud S , Filippakopoulos P , Goudarzi A , Ibrahim Z , Panne D , Rousseaux S , Zhao Y , Khochbin S .

Nuclear protein in testis (Nut) is a universal oncogenic driver in the highly aggressive NUT midline carcinoma, whose physiological function in male germ cells has been unclear. Here we show that expression of Nut is normally restricted to post-meiotic spermatogenic cells, where its presence triggers p300-dependent genome-wide histone H4 hyperacetylation, which is essential for the completion of histone-to-protamine exchange. Accordingly, the inactivation of Nut induces male sterility with spermatogenesis arrest at the histone-removal stage. Nut uses p300 and/or CBP to enhance acetylation of H4 at both K5 and K8, providing binding sites for the first bromodomain of Brdt, the testis-specific member of the BET family, which subsequently mediates genome-wide histone removal. Altogether, our data reveal the detailed molecular basis of the global histone hyperacetylation wave, which occurs before the final compaction of the male genome.

PubMed ID: 30257209
Article link: Cell Rep
Grant support: [+]

Species referenced: Xenopus
Genes referenced: crebbp