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Cells January 1, 2020; 9 (1):

Hydrogen Sulfide Impairs Meiosis Resumption in Xenopuslaevis Oocytes.

Gelaude A , Slaby S , Cailliau K , Marin M , Lescuyer-Rousseau A , Molinaro C , Nevoral J , Kučerová-Chrpová V , Sedmikova M , Petr J , Martoriati A , Bodart JF .

The role of hydrogen sulfide (H2S) is addressed in Xenopuslaevis oocytes. Three enzymes involved in H2S metabolism, cystathionine β-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate sulfurtransferase, were detected in prophase I and metaphase II-arrested oocytes and drove an acceleration of oocyte meiosis resumption when inhibited. Moreover, meiosis resumption is associated with a significant decrease in endogenous H2S. On another hand, a dose-dependent inhibition was obtained using the H2S donor, NaHS (1 and 5 mM). NaHS impaired translation. NaHS did not induce the dissociation of the components of the M-phase promoting factor (MPF), cyclin B and Cdk1, nor directly impacted the MPF activity. However, the M-phase entry induced by microinjection of metaphase II MPF-containing cytoplasm was diminished, suggesting upstream components of the MPF auto-amplification loop were sensitive to H2S. Superoxide dismutase and catalase hindered the effects of NaHS, and this sensitivity was partially dependent on the production of reactive oxygen species (ROS). In contrast to other species, no apoptosis was promoted. These results suggest a contribution of H2S signaling in the timing of amphibian oocytes meiosis resumption.

PubMed ID: 31963573
PMC ID: PMC7017156
Article link: Cells

Species referenced: Xenopus laevis
Genes referenced: cat.1 cdc25c cdk1 epha4 kcnj3 mapk1 mos mpst myc shb sod1

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References [+] :
Abrieu, The Polo-like kinase Plx1 is a component of the MPF amplification loop at the G2/M-phase transition of the cell cycle in Xenopus eggs. 1999, Pubmed, Xenbase