XB-ART-56927
Plant Cell
2020 Jul 01;327:2216-2236. doi: 10.1105/tpc.19.00578.
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STRESS INDUCED FACTOR 2 Regulates Arabidopsis Stomatal Immunity through Phosphorylation of the Anion Channel SLAC1.
Chan C
,
Panzeri D
,
Okuma E
,
Tõldsepp K
,
Wang YY
,
Louh GY
,
Chin TC
,
Yeh YH
,
Yeh HL
,
Yekondi S
,
Huang YH
,
Huang TY
,
Chiou TJ
,
Murata Y
,
Kollist H
,
Zimmerli L
.
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Upon recognition of microbes, pattern recognition receptors (PRRs) activate pattern-triggered immunity. FLAGELLIN SENSING2 (FLS2) and BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 (BAK1) form a typical PRR complex that senses bacteria. Here, we report that the kinase activity of the malectin-like receptor-like kinase STRESS INDUCED FACTOR 2 (SIF2) is critical for Arabidopsis (Arabidopsis thaliana) resistance to bacteria by regulating stomatal immunity. SIF2 physically associates with the FLS2-BAK1 PRR complex and interacts with and phosphorylates the guard cell SLOW ANION CHANNEL1 (SLAC1), which is necessary for abscisic acid (ABA)-mediated stomatal closure. SIF2 is also required for the activation of ABA-induced S-type anion currents in Arabidopsis protoplasts, and SIF2 is sufficient to activate SLAC1 anion channels in Xenopus oocytes. SIF2-mediated activation of SLAC1 depends on specific phosphorylation of Ser 65. This work reveals that SIF2 functions between the FLS2-BAK1 initial immunity receptor complex and the final actuator SLAC1 in stomatal immunity.
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Species referenced: Xenopus
Genes referenced: mlec nectin1
References [+] :
Alonso,
Genome-wide insertional mutagenesis of Arabidopsis thaliana.
2003, Pubmed
Alonso, Genome-wide insertional mutagenesis of Arabidopsis thaliana. 2003, Pubmed
Antolín-Llovera, Cleavage of the SYMBIOSIS RECEPTOR-LIKE KINASE ectodomain promotes complex formation with Nod factor receptor 5. 2014, Pubmed , Xenbase
Arnaud, A sophisticated network of signaling pathways regulates stomatal defenses to bacterial pathogens. 2015, Pubmed
Boller, A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. 2009, Pubmed
Boudsocq, Differential innate immune signalling via Ca(2+) sensor protein kinases. 2010, Pubmed
Brandt, Reconstitution of abscisic acid activation of SLAC1 anion channel by CPK6 and OST1 kinases and branched ABI1 PP2C phosphatase action. 2012, Pubmed , Xenbase
Chen, Homologue structure of the SLAC1 anion channel for closing stomata in leaves. 2010, Pubmed , Xenbase
Chinchilla, A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. 2007, Pubmed
Clough, Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. 1998, Pubmed
Curtis, A gateway cloning vector set for high-throughput functional analysis of genes in planta. 2003, Pubmed
Cutler, Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency. 2000, Pubmed
Dardick, Plant and animal pathogen recognition receptors signal through non-RD kinases. 2006, Pubmed
Desclos-Theveniau, The Arabidopsis lectin receptor kinase LecRK-V.5 represses stomatal immunity induced by Pseudomonas syringae pv. tomato DC3000. 2012, Pubmed
Dodds, Plant immunity: towards an integrated view of plant-pathogen interactions. 2010, Pubmed
Domínguez-Ferreras, An Overdose of the Arabidopsis Coreceptor BRASSINOSTEROID INSENSITIVE1-ASSOCIATED RECEPTOR KINASE1 or Its Ectodomain Causes Autoimmunity in a SUPPRESSOR OF BIR1-1-Dependent Manner. 2015, Pubmed
Geiger, Activity of guard cell anion channel SLAC1 is controlled by drought-stress signaling kinase-phosphatase pair. 2009, Pubmed , Xenbase
Girardin, Intracellular vs extracellular recognition of pathogens--common concepts in mammals and flies. 2002, Pubmed
Gust, Bacteria-derived peptidoglycans constitute pathogen-associated molecular patterns triggering innate immunity in Arabidopsis. 2007, Pubmed
Guzel Deger, Guard cell SLAC1-type anion channels mediate flagellin-induced stomatal closure. 2015, Pubmed
Gómez-Gómez, FLS2: an LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis. 2000, Pubmed
Gómez-Gómez, A single locus determines sensitivity to bacterial flagellin in Arabidopsis thaliana. 1999, Pubmed
Ha, Brassinosteroids modulate ABA-induced stomatal closure in Arabidopsis. 2016, Pubmed
Hanks, Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification. 1995, Pubmed
Heese, The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants. 2007, Pubmed
Hok, An Arabidopsis (malectin-like) leucine-rich repeat receptor-like kinase contributes to downy mildew disease. 2011, Pubmed
Hua, A plasma membrane receptor kinase, GHR1, mediates abscisic acid- and hydrogen peroxide-regulated stomatal movement in Arabidopsis. 2012, Pubmed , Xenbase
Huang, The Arabidopsis LecRK-VI.2 associates with the pattern-recognition receptor FLS2 and primes Nicotiana benthamiana pattern-triggered immunity. 2014, Pubmed
Huang, Arabidopsis thaliana transgenics overexpressing IBR3 show enhanced susceptibility to the bacterium Pseudomonas syringae. 2013, Pubmed
Huang, Enhancing crop innate immunity: new promising trends. 2014, Pubmed
Häweker, Pattern recognition receptors require N-glycosylation to mediate plant immunity. 2010, Pubmed
Johnson, Active and inactive protein kinases: structural basis for regulation. 1996, Pubmed
Kadota, Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 during plant immunity. 2014, Pubmed
Kadota, Immunoprecipitation of Plasma Membrane Receptor-Like Kinases for Identification of Phosphorylation Sites and Associated Proteins. 2016, Pubmed
Knighton, Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. 1991, Pubmed
Kong, Two Redundant Receptor-Like Cytoplasmic Kinases Function Downstream of Pattern Recognition Receptors to Regulate Activation of SA Biosynthesis. 2016, Pubmed
Korasick, Novel functions of Stomatal Cytokinesis-Defective 1 (SCD1) in innate immune responses against bacteria. 2010, Pubmed
Lee, Oligogalacturonic acid and chitosan reduce stomatal aperture by inducing the evolution of reactive oxygen species from guard cells of tomato and Commelina communis. 1999, Pubmed
Lee, Phosphorylation of the Plant Immune Regulator RPM1-INTERACTING PROTEIN4 Enhances Plant Plasma Membrane H⁺-ATPase Activity and Inhibits Flagellin-Triggered Immune Responses in Arabidopsis. 2015, Pubmed
Lee, A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. 2009, Pubmed , Xenbase
Lee, Bimolecular fluorescence complementation for imaging protein interactions in plant hosts of microbial pathogens. 2014, Pubmed
Leung, The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction. 1997, Pubmed
Li, The FLS2-associated kinase BIK1 directly phosphorylates the NADPH oxidase RbohD to control plant immunity. 2014, Pubmed
Li, Single-molecule analysis of PIP2;1 dynamics and partitioning reveals multiple modes of Arabidopsis plasma membrane aquaporin regulation. 2011, Pubmed
Liang, Arabidopsis heterotrimeric G proteins regulate immunity by directly coupling to the FLS2 receptor. 2016, Pubmed
Lim, Arabidopsis abscisic acid receptors play an important role in disease resistance. 2015, Pubmed
Lim, Function of ABA in Stomatal Defense against Biotic and Drought Stresses. 2015, Pubmed
Lozano-Durán, The bacterial effector HopM1 suppresses PAMP-triggered oxidative burst and stomatal immunity. 2014, Pubmed
Lu, A receptor-like cytoplasmic kinase, BIK1, associates with a flagellin receptor complex to initiate plant innate immunity. 2010, Pubmed
Macho, Plant PRRs and the activation of innate immune signaling. 2014, Pubmed
Mbengue, Clathrin-dependent endocytosis is required for immunity mediated by pattern recognition receptor kinases. 2016, Pubmed
Melotto, Plant stomata function in innate immunity against bacterial invasion. 2006, Pubmed
Monaghan, Plant pattern recognition receptor complexes at the plasma membrane. 2012, Pubmed
Montillet, An abscisic acid-independent oxylipin pathway controls stomatal closure and immune defense in Arabidopsis. 2013, Pubmed
Munemasa, Mechanisms of abscisic acid-mediated control of stomatal aperture. 2015, Pubmed
Murata, Diverse stomatal signaling and the signal integration mechanism. 2015, Pubmed
Mustilli, Arabidopsis OST1 protein kinase mediates the regulation of stomatal aperture by abscisic acid and acts upstream of reactive oxygen species production. 2002, Pubmed
Negi, CO2 regulator SLAC1 and its homologues are essential for anion homeostasis in plant cells. 2008, Pubmed
Nühse, Microbial elicitors induce activation and dual phosphorylation of the Arabidopsis thaliana MAPK 6. 2000, Pubmed
Pei, Differential abscisic acid regulation of guard cell slow anion channels in Arabidopsis wild-type and abi1 and abi2 mutants. 1997, Pubmed
Pei, Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. 2000, Pubmed
Petutschnig, The lysin motif receptor-like kinase (LysM-RLK) CERK1 is a major chitin-binding protein in Arabidopsis thaliana and subject to chitin-induced phosphorylation. 2010, Pubmed
Postel, The multifunctional leucine-rich repeat receptor kinase BAK1 is implicated in Arabidopsis development and immunity. 2010, Pubmed
Ranf, Interplay between calcium signalling and early signalling elements during defence responses to microbe- or damage-associated molecular patterns. 2011, Pubmed
Robatzek, Ligand-induced endocytosis of the pattern recognition receptor FLS2 in Arabidopsis. 2006, Pubmed
Roux, The Arabidopsis leucine-rich repeat receptor-like kinases BAK1/SERK3 and BKK1/SERK4 are required for innate immunity to hemibiotrophic and biotrophic pathogens. 2011, Pubmed
Schroeder, GUARD CELL SIGNAL TRANSDUCTION. 2001, Pubmed
Schulze, Rapid heteromerization and phosphorylation of ligand-activated plant transmembrane receptors and their associated kinase BAK1. 2010, Pubmed
Schwessinger, Phosphorylation-dependent differential regulation of plant growth, cell death, and innate immunity by the regulatory receptor-like kinase BAK1. 2011, Pubmed
Shi, BR-SIGNALING KINASE1 physically associates with FLAGELLIN SENSING2 and regulates plant innate immunity in Arabidopsis. 2013, Pubmed
Sierla, The Receptor-like Pseudokinase GHR1 Is Required for Stomatal Closure. 2018, Pubmed , Xenbase
Singh, Lectin receptor kinases in plant innate immunity. 2013, Pubmed
Singh, The lectin receptor kinase-VI.2 is required for priming and positively regulates Arabidopsis pattern-triggered immunity. 2012, Pubmed
Sirichandra, Phosphorylation of the Arabidopsis AtrbohF NADPH oxidase by OST1 protein kinase. 2009, Pubmed
Spallek, ESCRT-I mediates FLS2 endosomal sorting and plant immunity. 2013, Pubmed
Sun, Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. 2013, Pubmed
Tena, Protein kinase signaling networks in plant innate immunity. 2011, Pubmed
Tsai, Priming for enhanced defence responses by specific inhibition of the Arabidopsis response to coronatine. 2011, Pubmed
Tsuda, Comparing signaling mechanisms engaged in pattern-triggered and effector-triggered immunity. 2010, Pubmed
Vahisalu, SLAC1 is required for plant guard cell S-type anion channel function in stomatal signalling. 2008, Pubmed , Xenbase
Vahisalu, Ozone-triggered rapid stomatal response involves the production of reactive oxygen species, and is controlled by SLAC1 and OST1. 2010, Pubmed
Vlad, Protein phosphatases 2C regulate the activation of the Snf1-related kinase OST1 by abscisic acid in Arabidopsis. 2009, Pubmed
Walter, Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. 2004, Pubmed
Wang, Sequential transphosphorylation of the BRI1/BAK1 receptor kinase complex impacts early events in brassinosteroid signaling. 2008, Pubmed
Winter, An "Electronic Fluorescent Pictograph" browser for exploring and analyzing large-scale biological data sets. 2007, Pubmed
Xiao, The N-terminal region of Pseudomonas type III effector AvrPtoB elicits Pto-dependent immunity and has two distinct virulence determinants. 2007, Pubmed
Yeh, The Arabidopsis Malectin-Like/LRR-RLK IOS1 Is Critical for BAK1-Dependent and BAK1-Independent Pattern-Triggered Immunity. 2016, Pubmed
Yekondi, Nonredundant functions of Arabidopsis LecRK-V.2 and LecRK-VII.1 in controlling stomatal immunity and jasmonate-mediated stomatal closure. 2018, Pubmed
Yoo, Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. 2007, Pubmed
Yuan, STRESS INDUCED FACTOR 2, a Leucine-Rich Repeat Kinase Regulates Basal Plant Pathogen Defense. 2018, Pubmed
Zeng, Plant stomata: a checkpoint of host immunity and pathogen virulence. 2010, Pubmed
Zeng, A prominent role of the flagellin receptor FLAGELLIN-SENSING2 in mediating stomatal response to Pseudomonas syringae pv tomato DC3000 in Arabidopsis. 2010, Pubmed
Zeng, A genetic screen reveals Arabidopsis stomatal and/or apoplastic defenses against Pseudomonas syringae pv. tomato DC3000. 2011, Pubmed
Zhang, Receptor-like cytoplasmic kinases integrate signaling from multiple plant immune receptors and are targeted by a Pseudomonas syringae effector. 2010, Pubmed
Zhang, Resistance gene N-mediated de novo synthesis and activation of a tobacco mitogen-activated protein kinase by tobacco mosaic virus infection. 1998, Pubmed
Zimmerli, beta-Aminobutyric acid-induced protection of Arabidopsis against the necrotrophic fungus Botrytis cinerea. 2001, Pubmed