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EMBO J
2003 Mar 03;225:1005-13. doi: 10.1093/emboj/cdg118.
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Switching between the two action modes of the dual-affinity nitrate transporter CHL1 by phosphorylation.
Liu KH
,
Tsay YF
.
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To counteract fluctuating nutrient environments, plants have evolved high- and low-affinity uptake systems. These two systems were traditionally thought to be genetically distinct, but, recently, two Arabidopsis transporters, AtKUP1 and CHL1, were shown to have dual affinities. However, little is known about how a dual-affinity transporter works and the advantages of having a dual-affinity transporter. This study demonstrates that, in the case of CHL1, switching between the two modes of action is regulated by phosphorylation at threonine residue 101; when phosphorylated, CHL1 functions as a high-affinity nitrate transporter, whereas, when dephosphorylated, it functions as a low-affinity nitrate transporter. This regulatory mechanism allows plants to change rapidly between high- and low-affinity nitrate uptake, which may be critical when competing for limited nitrogen. These results demonstrate yet another regulatory role of phosphorylation in plant physiology.
Anderson,
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
1992, Pubmed
Anderson,
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
1992,
Pubmed
Arabidopsis Genome Initiative,
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
2000,
Pubmed
Bibb,
Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons.
1999,
Pubmed
Blakely,
Biogenic amine transporters: regulation in flux.
2000,
Pubmed
Boll,
Expression cloning and functional characterization of the kidney cortex high-affinity proton-coupled peptide transporter.
1996,
Pubmed
,
Xenbase
Béguin,
PKA-mediated phosphorylation of the human K(ATP) channel: separate roles of Kir6.2 and SUR1 subunit phosphorylation.
1999,
Pubmed
,
Xenbase
Cahalan,
Cell biology. Channels as enzymes.
2001,
Pubmed
Colón-Carmona,
Aux/IAA proteins are phosphorylated by phytochrome in vitro.
2000,
Pubmed
Covitz,
Membrane topology of the human dipeptide transporter, hPEPT1, determined by epitope insertions.
1998,
Pubmed
DeMaria,
Calmodulin bifurcates the local Ca2+ signal that modulates P/Q-type Ca2+ channels.
2001,
Pubmed
Esguerra,
Cloned Ca(2+)-dependent K+ channel modulated by a functionally associated protein kinase.
1994,
Pubmed
,
Xenbase
Fei,
Expression cloning of a mammalian proton-coupled oligopeptide transporter.
1994,
Pubmed
,
Xenbase
Fu,
AtKuP1: a dual-affinity K+ transporter from Arabidopsis.
1998,
Pubmed
Fuglsang,
Binding of 14-3-3 protein to the plasma membrane H(+)-ATPase AHA2 involves the three C-terminal residues Tyr(946)-Thr-Val and requires phosphorylation of Thr(947).
1999,
Pubmed
Gong,
Differential stimulation of PKC phosphorylation of potassium channels by ZIP1 and ZIP2.
1999,
Pubmed
Guo,
The Arabidopsis dual-affinity nitrate transporter gene AtNRT1.1 (CHL1) is activated and functions in nascent organ development during vegetative and reproductive growth.
2001,
Pubmed
Hagting,
Membrane topology of the di- and tripeptide transport protein of Lactococcus lactis.
1997,
Pubmed
Hirsch,
A role for the AKT1 potassium channel in plant nutrition.
1998,
Pubmed
Huang,
CHL1 encodes a component of the low-affinity nitrate uptake system in Arabidopsis and shows cell type-specific expression in roots.
1996,
Pubmed
,
Xenbase
Huang,
Cloning and functional characterization of an Arabidopsis nitrate transporter gene that encodes a constitutive component of low-affinity uptake.
1999,
Pubmed
,
Xenbase
Kim,
AtKUP1: an Arabidopsis gene encoding high-affinity potassium transport activity.
1998,
Pubmed
,
Xenbase
Kwak,
Phosphorylation is required for alteration of kv1.5 K(+) channel function by the Kvbeta1.3 subunit.
1999,
Pubmed
Leibach,
Peptide transporters in the intestine and the kidney.
1996,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Liu,
CHL1 is a dual-affinity nitrate transporter of Arabidopsis involved in multiple phases of nitrate uptake.
1999,
Pubmed
,
Xenbase
Liu,
Molecular cloning of PEPT 2, a new member of the H+/peptide cotransporter family, from human kidney.
1995,
Pubmed
Maathuis,
Roles of higher plant K+ channels.
1997,
Pubmed
Maurel,
Phosphorylation regulates the water channel activity of the seed-specific aquaporin alpha-TIP.
1995,
Pubmed
,
Xenbase
Mehrens,
The affinity of the organic cation transporter rOCT1 is increased by protein kinase C-dependent phosphorylation.
2000,
Pubmed
Paulsen,
The POT family of transport proteins.
1994,
Pubmed
Perraud,
ADP-ribose gating of the calcium-permeable LTRPC2 channel revealed by Nudix motif homology.
2001,
Pubmed
Roche,
Ca2+ channel beta3 subunit enhances voltage-dependent relief of G-protein inhibition induced by muscarinic receptor activation and Gbetagamma.
1998,
Pubmed
,
Xenbase
Runnels,
TRP-PLIK, a bifunctional protein with kinase and ion channel activities.
2001,
Pubmed
Saito,
Molecular cloning and tissue distribution of rat peptide transporter PEPT2.
1996,
Pubmed
,
Xenbase
Santa-María,
The HAK1 gene of barley is a member of a large gene family and encodes a high-affinity potassium transporter.
1997,
Pubmed
Schiff,
Tyrosine-kinase-dependent recruitment of RGS12 to the N-type calcium channel.
2000,
Pubmed
Sentenac,
Cloning and expression in yeast of a plant potassium ion transport system.
1992,
Pubmed
Svennelid,
Phosphorylation of Thr-948 at the C terminus of the plasma membrane H(+)-ATPase creates a binding site for the regulatory 14-3-3 protein.
1999,
Pubmed
Touraine,
NO3- and ClO3- fluxes in the chl1-5 mutant of Arabidopsis thaliana. Does the CHL1-5 gene encode a low-affinity NO3- transporter?
1997,
Pubmed
Trueman,
Molecular cloning of higher plant homologues of the high-affinity nitrate transporters of Chlamydomonas reinhardtii and Aspergillus nidulans.
1996,
Pubmed
Tsay,
The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate-inducible nitrate transporter.
1993,
Pubmed
,
Xenbase
Wang,
Ca(2+)-independent reduction of N-methyl-D-aspartate channel activity by protein tyrosine phosphatase.
1996,
Pubmed
Wang,
The Arabidopsis CHL1 protein plays a major role in high-affinity nitrate uptake.
1998,
Pubmed
Williams,
TRANSPORTERS RESPONSIBLE FOR THE UPTAKE AND PARTITIONING OF NITROGENOUS SOLUTES.
2001,
Pubmed
Xu,
Phosphorylation of the ATP-sensitive, inwardly rectifying K+ channel, ROMK, by cyclic AMP-dependent protein kinase.
1996,
Pubmed
,
Xenbase
