XB-ART-5700
FEBS Lett
2003 Feb 27;5371-3:151-6. doi: 10.1016/s0014-5793(03)00115-7.
Show Gene links
Show Anatomy links
Hypertonic activation of phospholemman in solitary rat hepatocytes in primary culture.
???displayArticle.abstract???
Under hypertonic conditions, solitary rat hepatocytes in primary culture shrink and subsequently exhibit a distinct regulatory volume increase (RVI). Reverse-transcribed polymerase chain reaction and 5' and 3' RACE (rapid amplification of cDNA ends) techniques reveal that these cells express phospholemman (PLM). In whole-cell recordings, the hypertonic activation of a channel is observed that resembles PLM with respect to unitary conductance (600-700 pS), gating pattern, and non-selectivity for Na(+) over K(+). In Xenopus oocytes expressing hepatocyte PLM, hypertonic stress induces a non-selective cation conductance and noise analysis reveals the activation of a channel with a unitary conductance of approximately 700 pS. These results suggest a role of PLM in the RVI of rat hepatocytes.
???displayArticle.pubmedLink??? 12606048
???displayArticle.link??? FEBS Lett
Species referenced: Xenopus laevis
Genes referenced: cyp26a1 fxyd1