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Proc Natl Acad Sci U S A January 1, 2020; 117 (28): 16283-16291.
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Cofactor-enabled functional expression of fruit fly, honeybee, and bumblebee nicotinic receptors reveals picomolar neonicotinoid actions.

Ihara M , Furutani S , Shigetou S , Shimada S , Niki K , Komori Y , Kamiya M , Koizumi W , Magara L , Hikida M , Noguchi A , Okuhara D , Yoshinari Y , Kondo S , Tanimoto H , Niwa R , Sattelle DB , Matsuda K .

The difficulty of achieving robust functional expression of insect nicotinic acetylcholine receptors (nAChRs) has hampered our understanding of these important molecular targets of globally deployed neonicotinoid insecticides at a time when concerns have grown regarding the toxicity of this chemotype to insect pollinators. We show that thioredoxin-related transmembrane protein 3 (TMX3) is essential to enable robust expression in Xenopus laevis oocytes of honeybee (Apis mellifera) and bumblebee (Bombus terrestris) as well as fruit fly (Drosophila melanogaster) nAChR heteromers targeted by neonicotinoids and not hitherto robustly expressed. This has enabled the characterization of picomolar target site actions of neonicotinoids, findings important in understanding their toxicity.

PubMed ID: 32611810
PMC ID: PMC7368294
Article link: Proc Natl Acad Sci U S A

Genes referenced: gabarap lgals4.2

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References [+] :
Barbara, Study of nicotinic acetylcholine receptors on cultured antennal lobe neurones from adult honeybee brains. 2008, Pubmed