Maldonado-Hernández R , Quesada O , Colón-Sáez JO , Lasalde-Dominicci JA .

P20 GM103642 NIGMS NIH HHS , R01 GM098343 NIGMS NIH HHS , R25 GM061151 NIGMS NIH HHS , U54 MD007600 NIMHD NIH HHS

Graphical Abstract

Addona, Where does cholesterol act during activation of the nicotinic acetylcholine receptor? 1998, Pubmed

Addona, Where does cholesterol act during activation of the nicotinic acetylcholine receptor? 1998, Pubmed
Anholt, Functional equivalence of monomeric and dimeric forms of purified acetylcholine receptors from Torpedo californica in reconstituted lipid vesicles. 1980, Pubmed
Antharavally, Efficient removal of detergents from proteins and peptides in a spin column format. 2011, Pubmed
Antollini, Disclosure of discrete sites for phospholipid and sterols at the protein-lipid interface in native acetylcholine receptor-rich membrane. 1998, Pubmed
Arias, The high-affinity quinacrine binding site is located at a non-annular lipid domain of the nicotinic acetylcholine receptor. 1997, Pubmed
Asmar-Rovira, Biophysical and ion channel functional characterization of the Torpedo californica nicotinic acetylcholine receptor in varying detergent-lipid environments. 2008, Pubmed
Baenziger, The Role of Cholesterol in the Activation of Nicotinic Acetylcholine Receptors. 2017, Pubmed
Baenziger, Effect of membrane lipid composition on the conformational equilibria of the nicotinic acetylcholine receptor. 2000, Pubmed
Baier, Disclosure of cholesterol recognition motifs in transmembrane domains of the human nicotinic acetylcholine receptor. 2011, Pubmed
Ballester, Up-regulation of the neuronal nicotinic receptor α7 by HIV glycoprotein 120: potential implications for HIV-associated neurocognitive disorder. 2012, Pubmed
Barrantes, A membrane-associated creatine kinase (EC 2.7.3.2) identified as an acidic species of the non-receptor, peripheral nu-proteins in Torpedo acetylcholine receptor membranes. 1983, Pubmed
Barrantes, Lipid matters: nicotinic acetylcholine receptor-lipid interactions (Review). 2002, Pubmed
Barrantes, Structural basis for lipid modulation of nicotinic acetylcholine receptor function. 2004, Pubmed
Basak, Cryo-EM structure of 5-HT3A receptor in its resting conformation. 2018, Pubmed , Xenbase
Bhushan, Differential scanning calorimetry and Fourier transform infrared analysis of lipid-protein interactions involving the nicotinic acetylcholine receptor. 1990, Pubmed
Bhushan, Correlation of phospholipid structure with functional effects on the nicotinic acetylcholine receptor. A modulatory role for phosphatidic acid. 1993, Pubmed
Bousse, Protein sizing on a microchip. 2001, Pubmed
Bowe, Identification and purification of an agrin receptor from Torpedo postsynaptic membranes: a heteromeric complex related to the dystroglycans. 1994, Pubmed
Brannigan, Embedded cholesterol in the nicotinic acetylcholine receptor. 2008, Pubmed
Brejc, Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors. 2001, Pubmed
Bukiya, Common structural features of cholesterol binding sites in crystallized soluble proteins. 2017, Pubmed
Burger, Regulation of receptor function by cholesterol. 2000, Pubmed
Butler, FTIR analysis of nicotinic acetylcholine receptor secondary structure in reconstituted membranes. 1993, Pubmed
Báez-Pagán, Heterogeneous Inhibition in Macroscopic Current Responses of Four Nicotinic Acetylcholine Receptor Subtypes by Cholesterol Enrichment. 2016, Pubmed , Xenbase
Cang, Mapping the functional binding sites of cholesterol in β2-adrenergic receptor by long-time molecular dynamics simulations. 2013, Pubmed
Capó-Vélez, The alpha7-nicotinic receptor contributes to gp120-induced neurotoxicity: implications in HIV-associated neurocognitive disorders. 2018, Pubmed
Carr, The 43-kilodalton protein of Torpedo nicotinic postsynaptic membranes: purification and determination of primary structure. 1987, Pubmed
Celie, Nicotine and carbamylcholine binding to nicotinic acetylcholine receptors as studied in AChBP crystal structures. 2004, Pubmed
Changeux, The nicotinic acetylcholine receptor: the founding father of the pentameric ligand-gated ion channel superfamily. 2012, Pubmed
Cheng, Anionic lipid and cholesterol interactions with alpha4beta2 nAChR: insights from MD simulations. 2009, Pubmed
Cherezov, LCP-FRAP Assay for Pre-Screening Membrane Proteins for in Meso Crystallization. 2008, Pubmed
Cherezov, Lipidic cubic phase technologies for membrane protein structural studies. 2011, Pubmed
Cherezov, High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor. 2007, Pubmed
Cohen, Purification from Torpedo marmorata electric tissue of membrane fragments particularly rich in cholinergic receptor protein. 1972, Pubmed
Conti-Tronconi, Brain and muscle nicotinic acetylcholine receptors are different but homologous proteins. 1985, Pubmed
Corringer, Structure and pharmacology of pentameric receptor channels: from bacteria to brain. 2012, Pubmed
Criado, Functional properties of the acetylcholine receptor incorporated in model lipid membranes. Differential effects of chain length and head group of phospholipids on receptor affinity states and receptor-mediated ion translocation. 1984, Pubmed
Criado, Effects of lipids on acetylcholine receptor. Essential need of cholesterol for maintenance of agonist-induced state transitions in lipid vesicles. 1982, Pubmed
Davis, Alcohol's actions on neuronal nicotinic acetylcholine receptors. 2006, Pubmed
Di Scala, Relevance of CARC and CRAC Cholesterol-Recognition Motifs in the Nicotinic Acetylcholine Receptor and Other Membrane-Bound Receptors. 2017, Pubmed
Edelstein, Molecular weight of the acetylcholine receptors of electric organs and the effect of Triton X-100. 1975, Pubmed
Fantini, Sphingolipid/cholesterol regulation of neurotransmitter receptor conformation and function. 2009, Pubmed
Fantini, Molecular mechanisms of protein-cholesterol interactions in plasma membranes: Functional distinction between topological (tilted) and consensus (CARC/CRAC) domains. 2016, Pubmed
Fernandez-Ballester, A role for cholesterol as a structural effector of the nicotinic acetylcholine receptor. 1994, Pubmed
Fernandez-Ballester, Protein stability and interaction of the nicotinic acetylcholine receptor with cholinergic ligands studied by Fourier-transform infrared spectroscopy. 1992, Pubmed
Finer-Moore, Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor. 1984, Pubmed
Fong, Correlation between acetylcholine receptor function and structural properties of membranes. 1986, Pubmed
Fong, Stabilization of acetylcholine receptor secondary structure by cholesterol and negatively charged phospholipids in membranes. 1987, Pubmed
Francis, The cholinergic hypothesis of Alzheimer's disease: a review of progress. 1999, Pubmed
Frost, Covalent immobilization of proteins to N-hydroxysuccinimide ester derivatives of agarose. Effect of protein charge on immobilization. 1981, Pubmed
Fujiwara, Dependence of α-helical and β-sheet amino acid propensities on the overall protein fold type. 2012, Pubmed
Gallegos, Ceramides modulate cell-surface acetylcholine receptor levels. 2008, Pubmed
Gangitano, Progesterone modulation of alpha5 nAChR subunits influences anxiety-related behavior during estrus cycle. 2009, Pubmed
Gharpure, Agonist Selectivity and Ion Permeation in the α3β4 Ganglionic Nicotinic Receptor. 2019, Pubmed
Grouleff, The influence of cholesterol on membrane protein structure, function, and dynamics studied by molecular dynamics simulations. 2015, Pubmed
Grutter, Structural reorganization of the acetylcholine binding site of the torpedo nicotinic receptor as revealed by dynamic photoaffinity labeling. 2002, Pubmed
Hamouda, Probing the structure of the affinity-purified and lipid-reconstituted torpedo nicotinic acetylcholine receptor. 2008, Pubmed
Hamouda, Cholesterol interacts with transmembrane alpha-helices M1, M3, and M4 of the Torpedo nicotinic acetylcholine receptor: photolabeling studies using [3H]Azicholesterol. 2006, Pubmed
Hamouda, Assessing the lipid requirements of the Torpedo californica nicotinic acetylcholine receptor. 2006, Pubmed
Hansen, Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive binding interfaces and conformations. 2005, Pubmed
Hassaine, X-ray structure of the mouse serotonin 5-HT3 receptor. 2014, Pubmed
Haviv, Neutral phospholipids stimulate Na,K-ATPase activity: a specific lipid-protein interaction. 2013, Pubmed
Hilf, X-ray structure of a prokaryotic pentameric ligand-gated ion channel. 2008, Pubmed
Hilf, Structure of a potentially open state of a proton-activated pentameric ligand-gated ion channel. 2009, Pubmed
Huang, Crystal structures of human glycine receptor α3 bound to a novel class of analgesic potentiators. 2017, Pubmed
Huang, Crystal structure of human glycine receptor-α3 bound to antagonist strychnine. 2015, Pubmed
Hucho, The acetylcholine receptor as part of a protein complex in receptor-enriched membrane fragments from Torpedo californica electric tissue. 1978, Pubmed
Ireland, Thermal melt circular dichroism spectroscopic studies for identifying stabilising amphipathic molecules for the voltage-gated sodium channel NavMs. 2018, Pubmed
Jamin, Characterization of the cholesterol recognition amino acid consensus sequence of the peripheral-type benzodiazepine receptor. 2005, Pubmed
Jones, Annular and nonannular binding sites for cholesterol associated with the nicotinic acetylcholine receptor. 1988, Pubmed
Khrustalev, The part of a long beta hairpin from the scrapie form of the human prion protein is reconstructed in the synthetic CC36 protein. 2016, Pubmed
Khrustalev, Amino acid content of beta strands and alpha helices depends on their flanking secondary structure elements. 2018, Pubmed
Koshy, Structural evidence for functional lipid interactions in the betaine transporter BetP. 2013, Pubmed
Kubalek, Location of subunits within the acetylcholine receptor by electron image analysis of tubular crystals from Torpedo marmorata. 1987, Pubmed
Kumar, Factors enhancing protein thermostability. 2000, Pubmed
Landau, Lipidic cubic phases: a novel concept for the crystallization of membrane proteins. 1996, Pubmed
Lasalde, Tryptophan substitutions at the lipid-exposed transmembrane segment M4 of Torpedo californica acetylcholine receptor govern channel gating. 1996, Pubmed , Xenbase
Lascombe, The 1.45 A resolution structure of the cryptogein-cholesterol complex: a close-up view of a sterol carrier protein (SCP) active site. 2002, Pubmed
Le Novère, Improved secondary structure predictions for a nicotinic receptor subunit: incorporation of solvent accessibility and experimental data into a two-dimensional representation. 1999, Pubmed
Li, Peripheral-type benzodiazepine receptor function in cholesterol transport. Identification of a putative cholesterol recognition/interaction amino acid sequence and consensus pattern. 1998, Pubmed
Lindstrom, Immunochemical similarities between subunits of acetylcholine receptors from Torpedo, Electrophorus, and mammalian muscle. 1979, Pubmed
Liu, Crystallization of membrane proteins in lipidic mesophases. 2011, Pubmed
Maldonado-Hernández, Biophysical characterization dataset of native nicotinic acetylcholine receptor in lipid-like detergent complexes. 2020, Pubmed
Marsal, Incorporation of acetylcholine receptors and Cl- channels in Xenopus oocytes injected with Torpedo electroplaque membranes. 1995, Pubmed , Xenbase
McCarthy, Effects of lipids and detergents on the conformation of the nicotinic acetylcholine receptor from Torpedo californica. 1992, Pubmed
Mehmood, Structural and Functional Basis for Lipid Synergy on the Activity of the Antibacterial Peptide ABC Transporter McjD. 2016, Pubmed
Miller, Crystal structure of a human GABAA receptor. 2014, Pubmed
Miyazawa, Structure and gating mechanism of the acetylcholine receptor pore. 2003, Pubmed
Miyazawa, Nicotinic acetylcholine receptor at 4.6 A resolution: transverse tunnels in the channel wall. 1999, Pubmed
Moore, On the conformation of the acetylcholine receptor protein from Torpedo nobiliana. 1974, Pubmed
Morales-Perez, X-ray structure of the human α4β2 nicotinic receptor. 2016, Pubmed
Méthot, Structure of both the ligand- and lipid-dependent channel-inactive states of the nicotinic acetylcholine receptor probed by FTIR spectroscopy and hydrogen exchange. 1995, Pubmed
Méthot, Secondary structure of the nicotinic acetylcholine receptor: implications for structural models of a ligand-gated ion channel. 1994, Pubmed
Narayanaswami, Protein-lipid interactions and Torpedo californica nicotinic acetylcholine receptor function. 2. Membrane fluidity and ligand-mediated alteration in the accessibility of gamma subunit cysteine residues to cholesterol. 1993, Pubmed
Ochoa, Reconstitution of acetylcholine receptor function in lipid vesicles of defined composition. 1983, Pubmed
Ontong, Effect of a cholesterol-rich lipid environment on the enzymatic activity of reconstituted hyaluronan synthase. 2014, Pubmed
Padilla-Morales, Assessment of the functionality and stability of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology. 2016, Pubmed , Xenbase
Padilla-Morales, Effects of lipid-analog detergent solubilization on the functionality and lipidic cubic phase mobility of the Torpedo californica nicotinic acetylcholine receptor. 2011, Pubmed
Padilla-Morales, Functionality and stability data of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology. 2016, Pubmed , Xenbase
Pfeiffer, Purification by affinity chromatography of the glycine receptor of rat spinal cord. 1982, Pubmed
Philip, Nicotinic acetylcholine receptors and depression: a review of the preclinical and clinical literature. 2010, Pubmed
Popot, Interaction of the acetylcholine (nicotinic) receptor protein from Torpedo marmorata electric organ with monolayers of pure lipids. 1978, Pubmed
Pyle, Structural Lipids Enable the Formation of Functional Oligomers of the Eukaryotic Purine Symporter UapA. 2018, Pubmed
Quesada, Uncovering the lipidic basis for the preparation of functional nicotinic acetylcholine receptor detergent complexes for structural studies. 2016, Pubmed
Raftery, The biochemistry of an acetylcholine receptor. 1974, Pubmed
Rahman, Structure of the Native Muscle-type Nicotinic Receptor and Inhibition by Snake Venom Toxins. 2020, Pubmed
Rankin, The cholesterol dependence of activation and fast desensitization of the nicotinic acetylcholine receptor. 1997, Pubmed
Ray, Neuronal nicotinic acetylcholine receptor subunits in autism: an immunohistochemical investigation in the thalamus. 2005, Pubmed
Rossman, The physiology of the nicotinic acetylcholine receptor and its importance in the administration of anesthesia. 2011, Pubmed
Ryan, Structural effects of neutral and anionic lipids on the nicotinic acetylcholine receptor. An infrared difference spectroscopy study. 1996, Pubmed
Saitoh, Conditions for the selective labelling of the 66 000 dalton chain of the acetylcholine receptor by the covalent non-competitive blocker 5-azido-[3H]trimethisoquin. 1980, Pubmed
Santiago, Probing the effects of membrane cholesterol in the Torpedo californica acetylcholine receptor and the novel lipid-exposed mutation alpha C418W in Xenopus oocytes. 2001, Pubmed , Xenbase
Sauguet, Structural basis for ion permeation mechanism in pentameric ligand-gated ion channels. 2013, Pubmed , Xenbase
Schick, Assembly of the m2 tetramer is strongly modulated by lipid chain length. 2010, Pubmed
Schofield, Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family. , Pubmed , Xenbase
Sharma, Nicotinic Receptors: Role in Addiction and Other Disorders of the Brain. 2008, Pubmed
Sobel, Large-scale purification of the acetylcholine-receptor protein in its membrane-bound and detergent-extracted forms from Torpedo marmorata electric organ. 1977, Pubmed
Sonntag, Mutual adaptation of a membrane protein and its lipid bilayer during conformational changes. 2011, Pubmed
Sunshine, Lipid modulation of nicotinic acetylcholine receptor function: the role of neutral and negatively charged lipids. 1992, Pubmed
Tashima, The effect of cholesterol and monosialoganglioside (GM1) on the release and aggregation of amyloid beta-peptide from liposomes prepared from brain membrane-like lipids. 2004, Pubmed
Thompson, The functional role of the αM4 transmembrane helix in the muscle nicotinic acetylcholine receptor probed through mutagenesis and coevolutionary analyses. 2020, Pubmed
Tian, GABA- and acetylcholine-related gene expression in blood correlate with tic severity and microarray evidence for alternative splicing in Tourette syndrome: a pilot study. 2011, Pubmed
Toyoshima, Ion channel of acetylcholine receptor reconstructed from images of postsynaptic membranes. 1988, Pubmed
Tsigelny, A model of the nicotinic receptor extracellular domain based on sequence identity and residue location. 1997, Pubmed
Unwin, Refined structure of the nicotinic acetylcholine receptor at 4A resolution. 2005, Pubmed
Unwin, Nicotinic acetylcholine receptor at 9 A resolution. 1993, Pubmed
Unwin, Segregation of lipids near acetylcholine-receptor channels imaged by cryo-EM. 2017, Pubmed
Wagner, The 87K postsynaptic membrane protein from Torpedo is a protein-tyrosine kinase substrate homologous to dystrophin. 1993, Pubmed
Wang, Modes of Cholesterol Binding in Membrane Proteins: A Joint Analysis of 73 Crystal Structures. 2019, Pubmed
Weill, Affinity-labeling of purified acetylcholine receptor from Torpedo californica. 1974, Pubmed
Wenz, A fluorescent derivatization method of proteins for the detection of low-level impurities by microchip capillary gel electrophoresis. 2010, Pubmed
Wilens, Neuronal nicotinic receptor agonists for the treatment of attention-deficit/hyperactivity disorder: focus on cognition. 2007, Pubmed
Xu, Development of an Automated High Throughput LCP-FRAP Assay to Guide Membrane Protein Crystallization in Lipid Mesophases. 2011, Pubmed
Zhou, Free cholesterol induces higher β-sheet content in Aβ peptide oligomers by aromatic interaction with Phe19. 2012, Pubmed
Zoli, Diversity of native nicotinic receptor subtypes in mammalian brain. 2015, Pubmed
daCosta, A lipid-dependent uncoupled conformation of the acetylcholine receptor. 2009, Pubmed
daCosta, Lipid-protein interactions at the nicotinic acetylcholine receptor. A functional coupling between nicotinic receptors and phosphatidic acid-containing lipid bilayers. 2002, Pubmed