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XB-ART-57609
Elife January 1, 2020; 9

Co-movement of astral microtubules, organelles and F-actin by dynein and actomyosin forces in frog egg cytoplasm.

Pelletier JF , Field CM , Fürthauer S , Sonnett M , Mitchison TJ .


Abstract
How bulk cytoplasm generates forces to separate post-anaphase microtubule (MT) asters in Xenopus laevis and other large eggs remains unclear. Previous models proposed that dynein-based, inward organelle transport generates length-dependent pulling forces that move centrosomes and MTs outwards, while other components of cytoplasm are static. We imaged aster movement by dynein and actomyosin forces in Xenopus egg extracts and observed outward co-movement of MTs, endoplasmic reticulum (ER), mitochondria, acidic organelles, F-actin, keratin, and soluble fluorescein. Organelles exhibited a burst of dynein-dependent inward movement at the growing aster periphery, then mostly halted inside the aster, while dynein-coated beads moved to the aster center at a constant rate, suggesting organelle movement is limited by brake proteins or other sources of drag. These observations call for new models in which all components of the cytoplasm comprise a mechanically integrated aster gel that moves collectively in response to dynein and actomyosin forces.

PubMed ID: 33284105
PMC ID: PMC7759381
Article link: Elife
Grant support: [+]

Genes referenced: abl1 dnai1 hook2 incenp mapre1 mapt
GO keywords: aster [+]


Article Images: [+] show captions
References [+] :
Basant, Spatiotemporal Regulation of RhoA during Cytokinesis. 2019, Pubmed