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STAR Protoc December 18, 2020; 1 (3): 100221.
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Microfluidic encapsulation of Xenopus laevis cell-free extracts using hydrogel photolithography.

Geisterfer ZM , Oakey J , Gatlin JC .

Cell-free extract derived from the eggs of the African clawed frog Xenopus laevis is a well-established model system that has been used historically in bulk aliquots. Here, we describe a microfluidic approach for isolating discrete, biologically relevant volumes of cell-free extract, with more expansive and precise control of extract shape compared with extract-oil emulsions. This approach is useful for investigating the mechanics of intracellular processes affected by cell geometry or cytoplasmic volume, including organelle scaling and positioning mechanisms. For complete details on the use and execution of this protocol, please refer to Geisterfer et al. (2020).

PubMed ID: 33377113
PMC ID: PMC7757658
Article link: STAR Protoc
Grant support: [+]

Genes referenced: dmd.2

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References [+] :
Debroy, Interfacially-mediated oxygen inhibition for precise and continuous poly(ethylene glycol) diacrylate (PEGDA) particle fabrication. 2018, Pubmed