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STAR Protoc December 18, 2020; 1 (3): 100151.

Affinity Purification of Label-free Tubulins from Xenopus Egg Extracts.

Reusch S , Biswas A , Hirst WG , Reber S .

Cytoplasmic extracts from unfertilized Xenopus eggs have made important contributions to our understanding of microtubule dynamics, spindle assembly, and scaling. Until recently, these in vitro studies relied on the use of heterologous tubulin. This protocol allows for the purification of physiologically relevant Xenopus tubulins in milligram yield, which are a complex mixture of isoforms with various post-translational modifications. The protocol is applicable to any cell or tissue of interest. For complete details on the use and execution of this protocol, please refer to Hirst et al. (2020).

PubMed ID: 33377045
PMC ID: PMC7757314
Article link: STAR Protoc

Genes referenced: ckap5 mrc1 nhs

Article Images: [+] show captions
References [+] :
Al-Bassam, Regulation of microtubule dynamics by TOG-domain proteins XMAP215/Dis1 and CLASP. 2012, Pubmed, Xenbase