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Proc Natl Acad Sci U S A February 18, 2003; 100 (4): 1639-44.
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Exonucleolytic degradation of double-stranded RNA by an activity in Xenopus laevis germinal vesicles.

Fruscoloni P , Zamboni M , Baldi MI , Tocchini-Valentini GP .

We have identified, in extracts from Xenopus laevis germinal vesicles, a 5'' exonuclease activity that cleaves double-stranded RNA (dsRNA). Features of the 5'' ends of dsRNAs determine whether the strands are symmetrically or asymmetrically degraded. The activity hydrolyzes in the 5'' to 3'' direction, releasing 5''-mononucleotides processively, favoring strands with 5''-monophosphate termini; molecules with capped ends are resistant to digestion. Because of its ability to processively digest dsRNA to mononucleotides, we have named the exonuclease Chipper, which could cooperate or compete with Dicer (an endonuclease that produces molecules with a 5''-phosphate) in the processing of dsRNA.

PubMed ID: 12578969
PMC ID: PMC149885
Article link: Proc Natl Acad Sci U S A

Species referenced: Xenopus laevis
Genes referenced: dicer1

References [+] :
Bass, Double-stranded RNA as a template for gene silencing. 2000, Pubmed