Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Uncleaved TFIIA is a substrate for taspase 1 and active in transcription.
Zhou H
,
Spicuglia S
,
Hsieh JJ
,
Mitsiou DJ
,
Høiby T
,
Veenstra GJ
,
Korsmeyer SJ
,
Stunnenberg HG
.
???displayArticle.abstract???
In higher eukaryotes, the large subunit of the general transcription factor TFIIA is encoded by the single TFIIAalphabeta gene and posttranslationally cleaved into alpha and beta subunits. The molecular mechanisms and biological significance of this proteolytic process have remained obscure. Here, we show that TFIIA is a substrate of taspase 1 as reported for the trithorax group mixed-lineage leukemia protein. We demonstrate that recombinant taspase 1 cleaves TFIIA in vitro. Transfected taspase 1 enhances cleavage of TFIIA, and RNA interference knockdown of endogenous taspase 1 diminishes cleavage of TFIIA in vivo. In taspase 1-/- MEF cells, only uncleaved TFIIA is detected. In Xenopus laevis embryos, knockdown of TFIIA results in phenotype and expression defects. Both defects can be rescued by expression of an uncleavable TFIIA mutant. Our study shows that uncleaved TFIIA is transcriptionally active and that cleavage of TFIIA does not serve to render TFIIA competent for transcription. We propose that cleavage fine tunes the transcription regulation of a subset of genes during differentiation and development.
Bleichenbacher,
Novel interactions between the components of human and yeast TFIIA/TBP/DNA complexes.
2003, Pubmed
Bleichenbacher,
Novel interactions between the components of human and yeast TFIIA/TBP/DNA complexes.
2003,
Pubmed
Catena,
Proteolytic cleavage of ALF into alpha- and beta-subunits that form homologous and heterologous complexes with somatic TFIIA and TRF2 in male germ cells.
2005,
Pubmed
Daser,
Extending the repertoire of the mixed-lineage leukemia gene MLL in leukemogenesis.
2004,
Pubmed
Geiger,
Crystal structure of the yeast TFIIA/TBP/DNA complex.
1996,
Pubmed
Han,
Expression of the germ cell-specific transcription factor ALF in Xenopus oocytes compensates for translational inactivation of the somatic factor TFIIA.
2003,
Pubmed
,
Xenbase
Hsieh,
Proteolytic cleavage of MLL generates a complex of N- and C-terminal fragments that confers protein stability and subnuclear localization.
2003,
Pubmed
Hsieh,
Taspase1: a threonine aspartase required for cleavage of MLL and proper HOX gene expression.
2003,
Pubmed
Høiby,
Cleavage and proteasome-mediated degradation of the basal transcription factor TFIIA.
2004,
Pubmed
Jallow,
Specialized and redundant roles of TBP and a vertebrate-specific TBP paralog in embryonic gene regulation in Xenopus.
2004,
Pubmed
,
Xenbase
Kobayashi,
A class of activation domains interacts directly with TFIIA and stimulates TFIIA-TFIID-promoter complex assembly.
1995,
Pubmed
Kobayashi,
DA-complex assembly activity required for VP16C transcriptional activation.
1998,
Pubmed
Kraemer,
TFIIA interacts with TFIID via association with TATA-binding protein and TAF40.
2001,
Pubmed
Lieberman,
Identification of functional targets of the Zta transcriptional activator by formation of stable preinitiation complex intermediates.
1994,
Pubmed
Lieberman,
Requirement for transcription factor IIA (TFIIA)-TFIID recruitment by an activator depends on promoter structure and template competition.
1997,
Pubmed
Mitsiou,
TAC, a TBP-sans-TAFs complex containing the unprocessed TFIIAalphabeta precursor and the TFIIAgamma subunit.
2000,
Pubmed
Mitsiou,
p300 is involved in formation of the TBP-TFIIA-containing basal transcription complex, TAC.
2003,
Pubmed
Nakamura,
ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation.
2002,
Pubmed
Ozer,
Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription.
1994,
Pubmed
Ranish,
Intermediates in formation and activity of the RNA polymerase II preinitiation complex: holoenzyme recruitment and a postrecruitment role for the TATA box and TFIIB.
1999,
Pubmed
Stargell,
The TBP-TFIIA interaction in the response to acidic activators in vivo.
1995,
Pubmed
Stargell,
TFIIA has activator-dependent and core promoter functions in vivo.
2000,
Pubmed
Sun,
Reconstitution of human TFIIA activity from recombinant polypeptides: a role in TFIID-mediated transcription.
1994,
Pubmed
Tan,
A modular polycistronic expression system for overexpressing protein complexes in Escherichia coli.
2001,
Pubmed
Tan,
Crystal structure of a yeast TFIIA/TBP/DNA complex.
1996,
Pubmed
Veenstra,
Translation of maternal TATA-binding protein mRNA potentiates basal but not activated transcription in Xenopus embryos at the midblastula transition.
1999,
Pubmed
,
Xenbase
Weideman,
Dynamic interplay of TFIIA, TBP and TATA DNA.
1997,
Pubmed
Yokomori,
Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex.
1993,
Pubmed
Yokomori,
Drosophila TFIIA directs cooperative DNA binding with TBP and mediates transcriptional activation.
1994,
Pubmed
Zorn,
The KH domain protein encoded by quaking functions as a dimer and is essential for notochord development in Xenopus embryos.
1997,
Pubmed
,
Xenbase
