Umair Z et al. (2020), Dusp1 modulates activin/smad2 mediated germ lay...

XB-ART-57742

Anim Cells Syst (Seoul) 2020 Nov 27;246:359-370. doi: 10.1080/19768354.2020.1847732.

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Dusp1 modulates activin/smad2 mediated germ layer specification via FGF signal inhibition in Xenopus embryos.

Umair Z , Kumar S , Rafiq K , Kumar V , Reman ZU , Lee SH , Kim S , Lee JY , Lee U , Kim J .

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Activin, a member of the transforming growth factor (TGF-β) superfamily, induces mesoderm, endoderm and neuro-ectoderm formation in Xenopus embryos. Despite several previous studies, the complicated gene regulatory network and genes involved in this induction await more elaboration. We identified expression of various fibroblast growth factor (FGF) genes in activin/smad2 treated animal cap explants (AC) of Xenopus embryos. Activin/smad2 increased fgf3/8 expression, which was reduced by co-injection of dominant negative activin receptor (DNAR) and dominant negative Fgf receptor (DNFR). Interestingly, activin/smad2 also increased expression of dual specificity phosphatase 1 (dusp1) which has been known to inhibit Fgf signaling. Dusp1 overexpression in dorsal marginal zone caused gastrulation defect and decreased Jnk/Erk phosphorylation as well as Smad1 linker region phosphorylation. Dusp1 decreased neural and organizer gene expression with increasing of endodermal and ventral gene expression in smad2 treated AC, indicating that dusp1 modulates germ layer specification. Dusp1 decreased neural gene expression in fgf8 treated AC, suggesting that Erk and/or Jnk phosphorylation may be involved in fgf8 induced neural induction. In addition, dusp1 decreased the reporter gene activities of activin response element (ARE) and increased it for bmp response element (BRE), indicating that dusp1 modulates two opposite morphogen signaling of dorsal (activin/Smad2) and ventral (bmp/Smad1) tracks, acting to fine tune the Fgf/Erk pathway.

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Genes referenced: acss2.2 babam2 bmp4 dusp1 egr2 fgf20 fgf3 fgf8 fgfr1 fgfr2 fgfr4 foxd4l1.1 foxg1 gata2 gbx2.2 gpx3 hoxb9 kcnk6 lnpep mapk1 mapk8 mix1 mixer ncam1 neurod1 nog otx2 pcdh8.2 pnhd rax smad1 smad2 smad4 sox17b.1 tal1 tbxt tgfb1 ventx1 zic3
GO keywords: BMP signaling pathway [+]

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	Figure 1. Ectopic expression of Activin, Smad2, and Fgfs in AC. All mRNAs (1 ng/embryo) were injected at the one-cell stage. The animal-caps were dissected at stage 8 and experiments were performed at stage 11 of Xenopus embryos. (A–C) DNAR and DNFR were injected separately and animal caps (AC) were dissected. ACs were treated with activin (25 ng/ml). The relative gene expressions were analyzed by RT-PCR and immunoprecipitation for pan Erk and pErk. (D–E) The AC were treated with activin. The specific gene expressions were analyzed by Microarray Affymetrix Xenopus Genome Gene Chip and RT-PCR of selected ESTs. (F) Stage-dependent spatial/ temporal expression of selected ESTs in whole embryos. (G–H) Fgf8a, Fgf8b and Smad2 were injected separately for RNA-Seq analysis and RT-PCR in AC.
	Figure 2. Overexpression of dusp1 mimics the DNFR function. (A) Dusp1 mRNA (3 ng/embryo) was injected at the 4 cell stage in dorsal half and harvested at stage 15 to identify the morphological change as compared to that of control (uninjected whole embryos). (B) Dusp1 (3 ng/embryo) was injected and harvested at stage 11-11.5. Immunoprecipitation was performed with pJnk, pErk, pSmad1C (c-terminal), pSmad1L (Linker region), pSmad2C (c-terminal) and α-tubulin was included as a control (α-tubulin being a housekeeping protein).
	Figure 3. Dusp1 inhibits Smad2 mediated dorsal mesoderm (organizer) in AC. (A–C) Smad2 mRNA (1 ng/embryo) was injected separately or co-injected with dusp1 (3 ng/embryo) at the one-cell stage, followed by dissection of the AC (AC) and harvested at stage 11-11.5. Relative gene expression was analyzed by RT-PCR. (C) BCI 10 µM (Dusp inhibitor) and DMSO as control, treated at stage 8.
	Figure 4. Dusp1 inhibits Fgf8 induced neural induction and modulates anterior-posterior patterning of neural tissue formation. (A–C) Fgf8a and fgf8b mRNA (1 ng/embryo) were injected separately or co-injected with dusp1 (3 ng/embryo) at the one-cell stage, followed by dissection of the AC (AC) at stage 8 and harvested at stage 11 and 24. RT-PCR was performed to examine the expression of target genes to compare with the control (uninjected AC), (C) BCI (Dusp1 inhibitor, 10 µM and DMSO as control) treatment at stage 8.
	Figure 5. Dusp1 regulates reporter activities of ARE, BRE and SCL promoter constructs. (A and B) The reporter ARE construct (40 pg/embryo) was injected with or without dusp1 (3 ng/embryo) at the one-cell stage and the embryos were grown until stage 11 and 18 for the relative reporter activity. (C) The reporter BRE construct (40 pg/embryo) was injected with or without dusp1 (3 ng/embryo) and/or fgf8b (1 ng/embryo) or together (with or without treatment with BCI and U0126) and the embryos at stage 11 to measure the relative reporter activity. (D) SCL promoter (40 pg/embryo) was injected with or without dusp1 (3 ng/embryo) at stage 11 to measure the relative promoter activity. The data are shown as mean ± S.E. of the values from at least three independent experiments. Differences were considered significant at P < 0.05.
	Figure 6. Schematic model: Dusp1 converts activin/Smad2 mediated neuro-ectoderm and dorsal mesoderm to ventral mesoderm and endoderm in AC of Xenopus embryos.

References [+] :

Asashima, Mesodermal induction in early amphibian embryos by activin A (erythroid differentiation factor). 1990, Pubmed, Xenbase