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MicroPubl Biol
2021 Feb 09;2021. doi: 10.17912/micropub.biology.000365.
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Actin polymerization is not required for the fast block to polyspermy in the African clawed frog, Xenopus laevis.
Tembo M
,
Sauer ML
,
Wisner BW
,
Beleny DO
,
Napolitano MA
,
Carlson AE
.
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Fertilization of an egg by multiple sperm presents one of the earliest and most prevalent obstacles to successful reproduction. Eggs employ multiple mechanisms to prevent sperm entry into the nascent zygote. The fast block to polyspermy uses a depolarization to inhibit sperm entry. For some external fertilizers, fertilization and the fast block require actin polymerization. Here we explored whether the fast block to polyspermy in the external fertilizer, Xenopus laevis, requires actin polymerization. Inseminating in the presence of inhibitor cytochalasin B, here we demonstrate that actin polymerization is not required for the fast block to polyspermy in X. laevis.
Figure 1 : Representative whole-cell recordings made during fertilization in control conditions (A) or 10 µg/ml cytochalasin B (B). Grey dashed lines denote 0 mV. Tukey box plot distributions of the resting (n = 10) and fertilization (n = 4) potentials (C). (D) Average percentage (± s.e.m.) of embryos inseminated under control conditions (red) or with 10 µg/ml cytochalasin B (blue), that developed normally to the 4-cell (NF 3) stage, with incomplete cytokinesis, or with abnormal cleavage furrows (n = 14), colors correspond to images shown in E. (E) Representative images of a NF 3 embryo, two cytochalasin B treated embryos with incomplete cytokinesis and a polyspermic embryo with abnormal cleavage furrows. *** denotes P < 0.001 and n.s denotes a P > 0.05 as determined by Student’s t-tests with the Bonferroni correction.
Bianchi,
Sperm Meets Egg: The Genetics of Mammalian Fertilization.
2016, Pubmed
Bianchi,
Sperm Meets Egg: The Genetics of Mammalian Fertilization.
2016,
Pubmed
Chun,
Early events of fertilization in sea urchin eggs are sensitive to actin-binding organic molecules.
2014,
Pubmed
Jaffe,
Fast block to polyspermy in sea urchin eggs is electrically mediated.
1976,
Pubmed
MacLean-Fletcher,
Mechanism of action of cytochalasin B on actin.
1980,
Pubmed
McCulloh,
Membrane depolarization facilitates sperm entry, large fertilization cone formation, and prolonged current responses in sea urchin oocytes.
1987,
Pubmed
Noguchi,
Reorganization of actin cytoskeleton at the growing end of the cleavage furrow of Xenopus egg during cytokinesis.
2001,
Pubmed
,
Xenbase
Silvestre,
Impact of marine drugs on cytoskeleton-mediated reproductive events.
2010,
Pubmed
Wolenski,
Effects of cytochalasins B and D on the fertilization of zebrafish (Brachydanio) eggs.
1988,
Pubmed
Wozniak,
The TMEM16A channel mediates the fast polyspermy block in Xenopus laevis.
2018,
Pubmed
,
Xenbase
Ziomek,
Polyspermy block of Spisula eggs is prevented by cytochalasin B.
1975,
Pubmed
