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XB-ART-57895
Exp Cell Res March 2, 2021; 402 (1): 112525.

Adherens junctions are involved in polarized contractile ring formation in dividing epithelial cells of Xenopus laevis embryos.

Hatte G , Prigent C , Tassan JP .


Abstract
Cells dividing in the plane of epithelial tissues proceed by polarized constriction of the actomyosin contractile ring, leading to asymmetric ingression of the plasma mem brane. Asymmetric cytokinesis results in the apical positioning of the actomyosin contractile ring and ultimately of the midbody. Studies have indicated that the contractile ring is associated with adherens junctions, whose role is to maintain epithelial tissue cohesion. However, it is yet unknown when the contractile ring becomes associated with adherens junctions in epithelial cells. Here, we examined contractile ring formation and activation in the epithelium of Xenopus embryos and explored the implication of adherens junctions in the contractile ring formation. We show that accumulation of proteins involved in contractile ring formation and activation is polarized, starting at apical cell-cell contacts at the presumptive division site and spreading within seconds towards the cell basal side. We also show that adherens junctions are involved in the kinetics of contractile ring formation. Our study reveals that the link between the adherens junctions and the contractile ring is established from the onset of cytokinesis.

PubMed ID: 33662366
Article link: Exp Cell Res


Species referenced: Xenopus laevis
Genes referenced: cdh3 ctnna1 ect2 tjp1
GO keywords: cytokinesis [+]
Antibodies: Actb Ab2 Cdh3 Ab1 Ctnna1 Ab4 Ctnnb1 Ab2 Ect2 Ab1 Kif23 Ab1 Myl9 Ab2 Tjp1 Ab2
Morpholinos: ctnna1 MO1 tjp1 MO1