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XB-ART-58104
Development January 1, 2020;
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Pinhead signaling regulates mesoderm heterogeneity via FGF receptor-dependent pathway.

Ossipova O , Itoh K , Radu A , Ezan J , Sokol SY .


Abstract
Among the three embryonic germ layers, mesoderm plays a central role in the establishment of the vertebrate body plan. Mesoderm is specified by secreted signaling proteins from the FGF, Nodal, BMP and Wnt families. No new classes of extracellular mesoderm-inducing factors have been identified in more than two decades. Here we show that the pinhead (pnhd) gene encodes a secreted protein that is essential for the activation of a subset of mesodermal markers in the Xenopus embryo. RNA sequencing revealed that many transcriptional targets of Pnhd are shared with those of the FGF pathway. Pnhd activity was accompanied by Erk phosphorylation and required FGF and Nodal but not Wnt signaling. We propose that during gastrulation Pnhd acts in the marginal zone to contribute to mesoderm heterogeneity via an FGF receptor-dependent positive feedback mechanism.

PubMed ID: 34004873
Article link: Development


Species referenced: Xenopus laevis
Genes referenced: bmp7.1 cdx1 cdx2 cdx4 cer1 col17a1 dand5 evx1 fgf8 fkbp9 foxc1 foxc2 foxd4l1.1 frzb gjc2 grem1 hoxd1 kcnk6 nodal3.1 nog nppb ntn3 pcdh8.2 pdgfra pnhd sebox slc26a9 tbx6 tbxt wnt8a zeb2 zg16
Antibodies: Ctnnb1 Ab9 FLAG Ab1 Smad1 Ab2
Morpholinos: pnhd MO4 pnhd MO5


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