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Soft Matter
2021 Jun 28;1724:5921-5931. doi: 10.1039/d1sm00045d.
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Confinement size determines the architecture of Ran-induced microtubule networks.
Gai Y
,
Cook B
,
Setru S
,
Stone HA
,
Petry S
.
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The organization of microtubules (MTs) is critical for cells during interphase and mitosis. During mitotic spindle assembly, MTs are made and organized around chromosomes in a process regulated by RanGTP. The role of RanGTP has been explored in Xenopus egg extracts, which are not limited by a cell membrane. Here, we investigated whether cell-sized confinements affect the assembly of RanGTP-induced MT networks in Xenopus egg extracts. We used microfluidics to encapsulate extracts within monodisperse extract-in-oil droplets. Importantly, we find that the architecture of Ran-induced MT networks depends on the droplet diameter and the Ran concentration, and differs from structures formed in bulk extracts. Our results highlight that both MT nucleation and physical confinement play critical roles in determining the spatial organization of the MT cytoskeleton.
Abate,
Glass coating for PDMS microfluidic channels by sol-gel methods.
2008, Pubmed
Abate,
Glass coating for PDMS microfluidic channels by sol-gel methods.
2008,
Pubmed
Alfaro-Aco,
Structural analysis of the role of TPX2 in branching microtubule nucleation.
2017,
Pubmed
,
Xenbase
Baumann,
Motor-mediated cortical versus astral microtubule organization in lipid-monolayered droplets.
2014,
Pubmed
,
Xenbase
Brugués,
Nucleation and transport organize microtubules in metaphase spindles.
2012,
Pubmed
,
Xenbase
Buendia,
A centrosomal antigen localized on intermediate filaments and mitotic spindle poles.
1990,
Pubmed
,
Xenbase
Cai,
Kinesin-14 family proteins HSET/XCTK2 control spindle length by cross-linking and sliding microtubules.
2009,
Pubmed
,
Xenbase
Carazo-Salas,
Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.
1999,
Pubmed
,
Xenbase
Carazo-Salas,
Ran-GTP coordinates regulation of microtubule nucleation and dynamics during mitotic-spindle assembly.
2001,
Pubmed
,
Xenbase
Cosentino Lagomarsino,
Microtubule organization in three-dimensional confined geometries: evaluating the role of elasticity through a combined in vitro and modeling approach.
2007,
Pubmed
Cyr,
Organization of cortical microtubules in plant cells.
1995,
Pubmed
Decker,
Autocatalytic microtubule nucleation determines the size and mass of Xenopus laevis egg extract spindles.
2018,
Pubmed
,
Xenbase
Desai,
Microtubule polymerization dynamics.
1997,
Pubmed
Dixit,
The cortical microtubule array: from dynamics to organization.
2004,
Pubmed
Dumont,
Acentrosomal spindle assembly and chromosome segregation during oocyte meiosis.
2012,
Pubmed
Foster,
Active contraction of microtubule networks.
2015,
Pubmed
,
Xenbase
Good,
Cytoplasmic volume modulates spindle size during embryogenesis.
2013,
Pubmed
,
Xenbase
Gruss,
Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity.
2001,
Pubmed
,
Xenbase
Guan,
A robust and tunable mitotic oscillator in artificial cells.
2018,
Pubmed
,
Xenbase
Hannak,
Investigating mitotic spindle assembly and function in vitro using Xenopus laevis egg extracts.
2006,
Pubmed
,
Xenbase
Hazel,
Changes in cytoplasmic volume are sufficient to drive spindle scaling.
2013,
Pubmed
,
Xenbase
Heald,
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase
Hoffmann,
Spatiotemporal control of microtubule nucleation and assembly using magnetic nanoparticles.
2013,
Pubmed
,
Xenbase
Juniper,
Spherical network contraction forms microtubule asters in confinement.
2018,
Pubmed
Kalab,
Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts.
2002,
Pubmed
,
Xenbase
Mitchison,
Growth, interaction, and positioning of microtubule asters in extremely large vertebrate embryo cells.
2012,
Pubmed
,
Xenbase
Montorzi,
Xenopus laevis embryo development: arrest of epidermal cell differentiation by the chelating agent 1,10-phenanthroline.
2000,
Pubmed
,
Xenbase
Nédélec,
Self-organization of microtubules and motors.
1997,
Pubmed
Oakey,
Microfluidic Encapsulation of Demembranated Sperm Nuclei in Xenopus Egg Extracts.
2018,
Pubmed
,
Xenbase
Ohba,
Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran.
1999,
Pubmed
,
Xenbase
Petry,
Mechanisms of Mitotic Spindle Assembly.
2016,
Pubmed
,
Xenbase
Petry,
Branching microtubule nucleation in Xenopus egg extracts mediated by augmin and TPX2.
2013,
Pubmed
,
Xenbase
Pinot,
Effects of confinement on the self-organization of microtubules and motors.
2009,
Pubmed
Roostalu,
Determinants of Polar versus Nematic Organization in Networks of Dynamic Microtubules and Mitotic Motors.
2018,
Pubmed
Surrey,
Physical properties determining self-organization of motors and microtubules.
2001,
Pubmed
Suzuki,
Spatial confinement of active microtubule networks induces large-scale rotational cytoplasmic flow.
2017,
Pubmed
,
Xenbase
Thawani,
Spatiotemporal organization of branched microtubule networks.
2019,
Pubmed
,
Xenbase
Verde,
Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein.
1991,
Pubmed
,
Xenbase
Wasteneys,
Spatial organization of plant cortical microtubules: close encounters of the 2D kind.
2009,
Pubmed
Weis,
Characterization of the nuclear protein import mechanism using Ran mutants with altered nucleotide binding specificities.
1996,
Pubmed
Wilde,
Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran.
1999,
Pubmed
,
Xenbase
Wittmann,
The spindle: a dynamic assembly of microtubules and motors.
2001,
Pubmed
Wühr,
Evidence for an upper limit to mitotic spindle length.
2008,
Pubmed
,
Xenbase
Xu,
Nucleation in polymers and soft matter.
2014,
Pubmed
Zhang,
Mechanisms of plant spindle formation.
2011,
Pubmed
Zhang,
Ran-GTP stabilises microtubule asters and inhibits nuclear assembly in Xenopus egg extracts.
1999,
Pubmed
,
Xenbase
Zheng,
The mitotic spindle matrix: a fibro-membranous lamin connection.
2006,
Pubmed
