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Biol Open July 15, 2021; 10 (7):

Rab7 is required for mesoderm patterning and gastrulation in Xenopus.

Kreis J , Wielath FM , Vick P .

Early embryogenesis requires tightly controlled temporal and spatial coordination of cellular behavior and signaling. Modulations are achieved at multiple levels, from cellular transcription to tissue-scale behavior. Intracellularly, the endolysosomal system emerges as an important regulator at different levels, but in vivo studies are rare. In the frog Xenopus, little is known about the developmental roles of endosomal regulators, or their potential involvement in signaling, especially for late endosomes. Here, we analyzed a hypothesized role of Rab7 in this context, a small GTPase known for its role as a late endosomal regulator. First, rab7 showed strong maternal expression. Following localized zygotic transcript enrichment in the mesodermal ring and neural plate, it was found in tailbud-stage neural ectoderm, notochord, pronephros, eyes and neural crest tissues. Inhibition resulted in strong axis defects caused by a requirement of rab7 for mesodermal patterning and correct gastrulation movements. To test a potential involvement in growth factor signaling, we analyzed early Wnt-dependent processes in the mesoderm. Our results suggest a selective requirement for ligand-induced Wnt activation, implicating a context-dependent role of Rab7.

PubMed ID: 34096568
Article link: Biol Open

Genes referenced: chd1 ctnnb1 dkk1 fgfr1 foxj1 gsc myf5 myod1 nodal3.1 nog not rab7a shh sox3 tbx6 ventx1.1 wnt8a
GO keywords: late endosome [+]
Antibodies: Itgb1 Ab1 Notochord Ab2
Morpholinos: rab7a MO1 rab7a MO2
gRNAs referenced: rab7a gRNA1 rab7a gRNA2

Article Images: [+] show captions