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Cell Rep July 6, 2021; 36 (1): 109340.

The Wnt/PCP formin Daam1 drives cell-cell adhesion during nephron development.

Krneta-Stankic V , Corkins ME , Paulucci-Holthauzen A , Kloc M , Gladden AB , Miller RK .

E-cadherin junctions facilitate assembly and disassembly of cell contacts that drive development and homeostasis of epithelial tissues. In this study, using Xenopus embryonic kidney and Madin-Darby canine kidney (MDCK) cells, we investigate the role of the Wnt/planar cell polarity (PCP) formin Daam1 (Dishevelled-associated activator of morphogenesis 1) in regulating E-cadherin-based intercellular adhesion. Using live imaging, we show that Daam1 localizes to newly formed cell contacts in the developing nephron. Furthermore, analyses of junctional filamentous actin (F-actin) upon Daam1 depletion indicate decreased microfilament localization and slowed turnover. We also show that Daam1 is necessary for efficient and timely localization of junctional E-cadherin, mediated by Daam1''s formin homology domain 2 (FH2). Finally, we establish that Daam1 signaling promotes organized movement of renal cells. This study demonstrates that Daam1 formin junctional activity is critical for epithelial tissue organization.

PubMed ID: 34233186
Article link: Cell Rep
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: cdh1 daam1 gapdh lhx1
Antibodies: Cdh1 Ab12 Daam1 Ab1 Daam1 Ab2 GFP Ab16 GFP Ab22 Gapdh Ab3 Lhx1 Ab4 RFP Ab4
Morpholinos: daam1 MO1

Article Images: [+] show captions