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XB-ART-58287
Sci Rep January 1, 2021; 11 (1): 14537.

Temporal transcriptomic profiling reveals dynamic changes in gene expression of Xenopus animal cap upon activin treatment.

Satou-Kobayashi Y , Kim JD , Fukamizu A , Asashima M .


Abstract
Activin, a member of the transforming growth factor-β (TGF-β) superfamily of proteins, induces various tissues from the amphibian presumptive ectoderm, called animal cap explants (ACs) in vitro. However, it remains unclear how and to what extent the resulting cells recapitulate in vivo development. To comprehensively understand whether the molecular dynamics during activin-induced ACs differentiation reflect the normal development, we performed time-course transcriptome profiling of Xenopus ACs treated with 50 ng/mL of activin A, which predominantly induced dorsal mesoderm. The number of differentially expressed genes (DEGs) in response to activin A increased over time, and totally 9857 upregulated and 6663 downregulated DEGs were detected. 1861 common upregulated DEGs among all Post_activin samples included several Spemann''s organizer genes. In addition, the temporal transcriptomes were clearly classified into four distinct groups in correspondence with specific features, reflecting stepwise differentiation into mesoderm derivatives, and a decline in the regulation of nuclear envelop and golgi. From the set of early responsive genes, we also identified the suppressor of cytokine signaling 3 (socs3) as a novel activin A-inducible gene. Our transcriptome data provide a framework to elucidate the transcriptional dynamics of activin-driven AC differentiation, reflecting the molecular characteristics of early normal embryogenesis.

PubMed ID: 34267234
Article link: Sci Rep


Species referenced: Xenopus laevis
Genes referenced: actc1 ccnd1 cdkn1a cer1 chrd.1 ctdnep1 dlx3 dnmt1 dusp1 eomes fgf3 fgf8 foxa4 fst fzd8 gata4 gsc lemd3 lhx1 map3k1 mix1 myf5 myog otx2 pou5f3.2 smad2 smad3 socs3 tp53 trim29 ventx1.2 wnt8a
GO keywords: ectoderm formation [+]

Phenotypes: Xla Wt + Activin

Article Images: [+] show captions