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Proc Natl Acad Sci U S A
2003 Jan 21;1002:751-6. doi: 10.1073/pnas.0134257100.
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Involvement of CjMDR1, a plant multidrug-resistance-type ATP-binding cassette protein, in alkaloid transport in Coptis japonica.
Shitan N
,
Bazin I
,
Dan K
,
Obata K
,
Kigawa K
,
Ueda K
,
Sato F
,
Forestier C
,
Yazaki K
.
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Alkaloids comprise one of the largest groups of plant secondary metabolites. Berberine, a benzylisoquinoline alkaloid, is preferentially accumulated in the rhizome of Coptis japonica, a ranunculaceous plant, whereas gene expression for berberine biosynthetic enzymes has been observed specifically in root tissues, which suggests that berberine synthesized in the root is transported to the rhizome, where there is high accumulation. We recently isolated a cDNA encoding a multidrug-resistance protein (MDR)-type ATP-binding cassette (ABC) transporter (Cjmdr1) from berberine-producing cultured C. japonica cells, which is highly expressed in the rhizome. Functional analysis of Cjmdr1 by using a Xenopus oocyte expression system showed that CjMDR1 transported berberine in an inward direction, resulting in a higher accumulation of berberine in Cjmdr1-injected oocytes than in the control. Typical inhibitors of ABC proteins, such as vanadate, nifedipine, and glibenclamide, as well as ATP depletion, clearly inhibited this CjMDR1-dependent berberine uptake, suggesting that CjMDR1 functioned as an ABC transporter. Conventional membrane separation methods showed that CjMDR1 was localized in the plasma membrane of C. japonica cells. In situ hybridization indicated that Cjmdr1 mRNA was expressed preferentially in xylem tissues of the rhizome. These findings strongly suggest that CjMDR1 is involved in the translocation of berberine from the root to the rhizome.
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987, Pubmed,
Xenbase
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
Davies,
Cloning and characterisation of a novel P-glycoprotein homologue from barley.
1997,
Pubmed
Decottignies,
ATPase and multidrug transport activities of the overexpressed yeast ABC protein Yor1p.
1998,
Pubmed
Gaedeke,
The Arabidopsis thaliana ABC transporter AtMRP5 controls root development and stomata movement.
2001,
Pubmed
Haas,
BiP (GRP78), an essential hsp70 resident protein in the endoplasmic reticulum.
1994,
Pubmed
Hayashi,
Ped3p is a peroxisomal ATP-binding cassette transporter that might supply substrates for fatty acid beta-oxidation.
2002,
Pubmed
Higgins,
ABC transporters: from microorganisms to man.
1992,
Pubmed
Hirata,
Saccharomyces cerevisiae YDR1, which encodes a member of the ATP-binding cassette (ABC) superfamily, is required for multidrug resistance.
1994,
Pubmed
Ito,
Transformation of intact yeast cells treated with alkali cations.
1983,
Pubmed
Iwasa,
Structure-activity relationships of protoberberines having antimicrobial activity.
1998,
Pubmed
Jasiński,
A plant plasma membrane ATP binding cassette-type transporter is involved in antifungal terpenoid secretion.
2001,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Liu,
Enhanced multispecificity of arabidopsis vacuolar multidrug resistance-associated protein-type ATP-binding cassette transporter, AtMRP2.
2001,
Pubmed
Lu,
AtMRP2, an Arabidopsis ATP binding cassette transporter able to transport glutathione S-conjugates and chlorophyll catabolites: functional comparisons with Atmrp1.
1998,
Pubmed
Martinoia,
Multifunctionality of plant ABC transporters--more than just detoxifiers.
2002,
Pubmed
Rea,
FROM VACUOLAR GS-X PUMPS TO MULTISPECIFIC ABC TRANSPORTERS.
1998,
Pubmed
Rentsch,
NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis.
1995,
Pubmed
Sakai,
Characterization of berberine transport into Coptis japonica cells and the involvement of ABC protein.
2002,
Pubmed
Sasaki,
A gene encoding multidrug resistance (MDR)-like protein is induced by aluminum and inhibitors of calcium flux in wheat.
2002,
Pubmed
Sato,
Specific differences in tolerance to exogenous berberine among plant cell cultures.
1990,
Pubmed
Servos,
Gene SNQ2 of Saccharomyces cerevisiae, which confers resistance to 4-nitroquinoline-N-oxide and other chemicals, encodes a 169 kDa protein homologous to ATP-dependent permeases.
1993,
Pubmed
Shih,
High-level expression and detection of ion channels in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Sidler,
Involvement of an ABC transporter in a developmental pathway regulating hypocotyl cell elongation in the light.
1998,
Pubmed
Sánchez-Fernández,
The Arabidopsis thaliana ABC protein superfamily, a complete inventory.
2001,
Pubmed
Theodoulou,
Plant ABC transporters.
2000,
Pubmed
Tommasini,
An ABC-transporter of Arabidopsis thaliana has both glutathione-conjugate and chlorophyll catabolite transport activity.
1998,
Pubmed
Ueda,
Expression of a full-length cDNA for the human "MDR1" gene confers resistance to colchicine, doxorubicin, and vinblastine.
1987,
Pubmed
Wang,
A potato cDNA encoding a homologue of mammalian multidrug resistant P-glycoprotein.
1996,
Pubmed
Yamada,
A family of transcripts encoding water channel proteins: tissue-specific expression in the common ice plant.
1995,
Pubmed
,
Xenbase
Yazaki,
Geranyl diphosphate:4-hydroxybenzoate geranyltransferase from Lithospermum erythrorhizon. Cloning and characterization of a ket enzyme in shikonin biosynthesis.
2002,
Pubmed
Yazaki,
A novel Coptis japonica multidrug-resistant protein preferentially expressed in the alkaloid-accumulating rhizome.
2001,
Pubmed
Zolman,
The Arabidopsis pxa1 mutant is defective in an ATP-binding cassette transporter-like protein required for peroxisomal fatty acid beta-oxidation.
2001,
Pubmed
