XB-ART-6018Can J Physiol Pharmacol November 1, 2002; 80 (11): 1119-23.
Show Gene links Show Anatomy links
Enhanced accumulation of constitutive heat shock protein mRNA is an initial response of eye tissue to mild hyperthermia in vivo in adult Xenopus laevis.
We have examined the effect of mild hyperthermia in vivo on heat shock transcription factor (HSF) binding activity and heat shock protein (hsp) gene expression in eye tissue of adult Xenopus laevis. A specific interaction between HSF and a synthetic oligonucleotide corresponding to the proximal heat shock element of the Xenopus hsp70B gene was greatly enhanced in eyes from hyperthermic animals compared with controls. Given these results, we examined the effect of hyperthermia in vivo on the expression of five hsp genes (hsp70, hsc70, BiP, hsp90, and hsp30) in eye tissue. Interestingly, at 28 degrees C constitutively expressed hsp genes hsc70, BiP, and hsp90 were strongly enhanced, with further accumulation at 30 degrees C. However, hsp70 and hsp30 mRNA accumulation were not detectable at 28 degrees C but were strongly induced at 30 degrees C. No enhancement of the relative levels of cytoskeletal actin mRNA was observed in the eye tissue of hyperthermic animals. These results suggest that one of the primary responses of eye tissue to hyperthermia in vivo is in the elevation of mRNAs encoding a set of constitutively expressed molecular chaperones.
PubMed ID: 12489931
Article link: Can J Physiol Pharmacol
Species referenced: Xenopus laevis
Genes referenced: actl6a hsp70 hsp90aa1.1 hspa1l hspa5 hspa8 osbpl8