Georges SA et al. (2002), p300-mediated tax transactivation from recombin...

XB-ART-7984

Mol Cell Biol 2002 Jan 01;221:127-37. doi: 10.1128/MCB.22.1.127-137.2002.

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p300-mediated tax transactivation from recombinant chromatin: histone tail deletion mimics coactivator function.

Georges SA , Kraus WL , Luger K , Nyborg JK , Laybourn PJ .

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Efficient transcription of the human T-cell leukemia virus type 1 (HTLV-1) genome requires Tax, a virally encoded oncogenic transcription factor, in complex with the cellular transcription factor CREB and the coactivators p300/CBP. To examine Tax transactivation in vitro, we used a chromatin assembly system that included recombinant core histones. The addition of Tax, CREB, and p300 to the HTLV-1 promoter assembled into chromatin activated transcription several hundredfold. Chromatin templates selectively lacking amino-terminal histone tails demonstrated enhanced transcriptional activation by Tax and CREB, with significantly reduced dependence on p300 and acetyl coenzyme A (acetyl-CoA). Interestingly, Tax/CREB activation from the tailless chromatin templates retained a substantial requirement for acetyl-CoA, indicating a role for acetyl-CoA beyond histone acetylation. These data indicate that during Tax transcriptional activation, the amino-terminal histone tails are the major targets of p300 and that tail deletion and acetylation are functionally equivalent.

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Species referenced: Xenopus laevis
Genes referenced: creb1 crebbp ep300 nr2e1

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