Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2001 Nov 15;2022:6424-33. doi: 10.1093/emboj/20.22.6424.
Show Gene links
Show Anatomy links
Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex.
Kataoka N
,
Diem MD
,
Kim VN
,
Yong J
,
Dreyfuss G
.
???displayArticle.abstract???
The RNA-binding protein Y14 binds preferentially to mRNAs produced by splicing and is a component of a multiprotein complex that assembles approximately 20 nucleotides upstream of exon-exon junctions. This complex probably has important functions in post-splicing events including nuclear export and nonsense-mediated decay of mRNA. We show that Y14 binds to two previously reported components, Aly/REF and RNPS1, and to the mRNA export factor TAP. Moreover, we identified magoh, a human homolog of the Drosophila mago nashi gene product, as a novel component of the complex. Magoh binds avidly and directly to Y14 and TAP, but not to other known components of the complex, and is found in Y14-containing mRNPs in vivo. Importantly, magoh also binds to mRNAs produced by splicing upstream (approximately 20 nucleotides) of exon- exon junctions and its binding to mRNA persists after export. These experiments thus reveal specific protein-protein interactions among the proteins of the splicing-dependent mRNP complex and suggest an important role for the highly evolutionarily conserved magoh protein in this complex.
Blencowe,
A coactivator of pre-mRNA splicing.
1998, Pubmed
Blencowe,
A coactivator of pre-mRNA splicing.
1998,
Pubmed
Bochnig,
A monoclonal antibody against 2,2,7-trimethylguanosine that reacts with intact, class U, small nuclear ribonucleoproteins as well as with 7-methylguanosine-capped RNAs.
1987,
Pubmed
Boswell,
Mutations in a newly identified Drosophila melanogaster gene, mago nashi, disrupt germ cell formation and result in the formation of mirror-image symmetrical double abdomen embryos.
1991,
Pubmed
Braun,
TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus.
1999,
Pubmed
,
Xenbase
Carter,
A splicing-dependent regulatory mechanism that detects translation signals.
1996,
Pubmed
Culbertson,
RNA surveillance. Unforeseen consequences for gene expression, inherited genetic disorders and cancer.
1999,
Pubmed
Czaplinski,
Should we kill the messenger? The role of the surveillance complex in translation termination and mRNA turnover.
1999,
Pubmed
Dreyfuss,
hnRNP proteins and the biogenesis of mRNA.
1993,
Pubmed
Frischmeyer,
Nonsense-mediated mRNA decay in health and disease.
1999,
Pubmed
Hanamura,
Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors.
1998,
Pubmed
Hentze,
A perfect message: RNA surveillance and nonsense-mediated decay.
1999,
Pubmed
Herold,
TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture.
2000,
Pubmed
Hilleren,
mRNA surveillance in eukaryotes: kinetic proofreading of proper translation termination as assessed by mRNP domain organization?
1999,
Pubmed
Jansen,
RNA-cytoskeletal associations.
1999,
Pubmed
Jansen,
mRNA localization: message on the move.
2001,
Pubmed
Kataoka,
Transportin-SR, a nuclear import receptor for SR proteins.
1999,
Pubmed
Kataoka,
Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm.
2000,
Pubmed
,
Xenbase
Kim,
The Y14 protein communicates to the cytoplasm the position of exon-exon junctions.
2001,
Pubmed
,
Xenbase
Kim,
Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex.
2001,
Pubmed
Krecic,
hnRNP complexes: composition, structure, and function.
1999,
Pubmed
Le Hir,
Pre-mRNA splicing alters mRNP composition: evidence for stable association of proteins at exon-exon junctions.
2000,
Pubmed
Le Hir,
The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions.
2000,
Pubmed
,
Xenbase
Li,
Nonsense surveillance in lymphocytes?
1998,
Pubmed
Li,
mag-1, a homolog of Drosophila mago nashi, regulates hermaphrodite germ-line sex determination in Caenorhabditis elegans.
2000,
Pubmed
Luo,
Splicing is required for rapid and efficient mRNA export in metazoans.
1999,
Pubmed
,
Xenbase
Lupas,
Predicting coiled coils from protein sequences.
1991,
Pubmed
Lykke-Andersen,
Human Upf proteins target an mRNA for nonsense-mediated decay when bound downstream of a termination codon.
2000,
Pubmed
Lykke-Andersen,
Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1.
2001,
Pubmed
Matsumoto,
Nuclear history of a pre-mRNA determines the translational activity of cytoplasmic mRNA.
1998,
Pubmed
,
Xenbase
Mayeda,
Purification and characterization of human RNPS1: a general activator of pre-mRNA splicing.
1999,
Pubmed
McGarvey,
The acute myeloid leukemia-associated protein, DEK, forms a splicing-dependent interaction with exon-product complexes.
2000,
Pubmed
Mendell,
Novel Upf2p orthologues suggest a functional link between translation initiation and nonsense surveillance complexes.
2000,
Pubmed
Michael,
A nuclear export signal in hnRNP A1: a signal-mediated, temperature-dependent nuclear protein export pathway.
1995,
Pubmed
Micklem,
The mago nashi gene is required for the polarisation of the oocyte and the formation of perpendicular axes in Drosophila.
1997,
Pubmed
Mingot,
Importin 13: a novel mediator of nuclear import and export.
2001,
Pubmed
Nagy,
A rule for termination-codon position within intron-containing genes: when nonsense affects RNA abundance.
1998,
Pubmed
Nakielny,
Nup153 is an M9-containing mobile nucleoporin with a novel Ran-binding domain.
1999,
Pubmed
Newmark,
mago nashi mediates the posterior follicle cell-to-oocyte signal to organize axis formation in Drosophila.
1997,
Pubmed
,
Xenbase
Newmark,
The mago nashi locus encodes an essential product required for germ plasm assembly in Drosophila.
1994,
Pubmed
Ohno,
A human RNA helicase-like protein, HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.
1996,
Pubmed
Oleynikov,
RNA localization: different zipcodes, same postman?
1998,
Pubmed
,
Xenbase
Pellizzoni,
A novel function for SMN, the spinal muscular atrophy disease gene product, in pre-mRNA splicing.
1998,
Pubmed
Piñol-Roma,
Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm.
1992,
Pubmed
,
Xenbase
Rodrigues,
REF proteins mediate the export of spliced and unspliced mRNAs from the nucleus.
2001,
Pubmed
,
Xenbase
Serin,
Identification and characterization of human orthologues to Saccharomyces cerevisiae Upf2 protein and Upf3 protein (Caenorhabditis elegans SMG-4).
2001,
Pubmed
Shyu,
The double lives of shuttling mRNA binding proteins.
2000,
Pubmed
Siomi,
Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins.
1997,
Pubmed
Stutz,
REF, an evolutionary conserved family of hnRNP-like proteins, interacts with TAP/Mex67p and participates in mRNA nuclear export.
2000,
Pubmed
,
Xenbase
Zhang,
Intron function in the nonsense-mediated decay of beta-globin mRNA: indications that pre-mRNA splicing in the nucleus can influence mRNA translation in the cytoplasm.
1998,
Pubmed
Zhang,
At least one intron is required for the nonsense-mediated decay of triosephosphate isomerase mRNA: a possible link between nuclear splicing and cytoplasmic translation.
1998,
Pubmed
Zhao,
The mammalian homologue of mago nashi encodes a serum-inducible protein.
1998,
Pubmed
Zhao,
MAGOH interacts with a novel RNA-binding protein.
2000,
Pubmed
Zhou,
The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans.
2000,
Pubmed
