Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay.
Le Hir H
,
Gatfield D
,
Izaurralde E
,
Moore MJ
.
???displayArticle.abstract???
We recently reported that spliceosomes alter messenger ribonucleoprotein particle (mRNP) composition by depositing several proteins 20-24 nucleotides upstream of mRNA exon-exon junctions. When assembled in vitro, this so-called 'exon-exon junction complex' (EJC) contains at least five proteins: SRm160, DEK, RNPS1, Y14 and REF. To better investigate its functional attributes, we now describe a method for generating spliced mRNAs both in vitro and in vivo that either do or do not carry the EJC. Analysis of these mRNAs in Xenopus laevis oocytes revealed that this complex is the species responsible for enhancing nucleocytoplasmic export of spliced mRNAs. It does so by providing a strong binding site for the mRNA export factors REF and TAP/p15. Moreover, by serving as an anchoring point for the factors Upf2 and Upf3, the EJC provides a direct link between splicing and nonsense-mediated mRNA decay. Finally, we show that the composition of the EJC is dynamic in vivo and is subject to significant evolution upon mRNA export to the cytoplasm.
Applequist,
Cloning and characterization of HUPF1, a human homolog of the Saccharomyces cerevisiae nonsense mRNA-reducing UPF1 protein.
1997, Pubmed
Applequist,
Cloning and characterization of HUPF1, a human homolog of the Saccharomyces cerevisiae nonsense mRNA-reducing UPF1 protein.
1997,
Pubmed
Bachi,
The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates.
2000,
Pubmed
,
Xenbase
Blencowe,
A coactivator of pre-mRNA splicing.
1998,
Pubmed
Braun,
Overexpression of TAP/p15 heterodimers bypasses nuclear retention and stimulates nuclear mRNA export.
2001,
Pubmed
,
Xenbase
Braun,
TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus.
1999,
Pubmed
,
Xenbase
Carter,
A splicing-dependent regulatory mechanism that detects translation signals.
1996,
Pubmed
Chanfreau,
Trans-complementation of the second step of pre-mRNA splicing by exogenous 5' exons.
1999,
Pubmed
Cheng,
Introns are cis effectors of the nonsense-codon-mediated reduction in nuclear mRNA abundance.
1994,
Pubmed
Conti,
Nucleocytoplasmic transport enters the atomic age.
2001,
Pubmed
Culbertson,
RNA surveillance. Unforeseen consequences for gene expression, inherited genetic disorders and cancer.
1999,
Pubmed
Czaplinski,
The surveillance complex interacts with the translation release factors to enhance termination and degrade aberrant mRNAs.
1998,
Pubmed
Duchêne,
Molecular consequences of truncations of the first exon for in vitro splicing of yeast actin pre-mRNA.
1988,
Pubmed
Grüter,
TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.
1998,
Pubmed
,
Xenbase
He,
Upf1p, Nmd2p, and Upf3p are interacting components of the yeast nonsense-mediated mRNA decay pathway.
1997,
Pubmed
Hentze,
A perfect message: RNA surveillance and nonsense-mediated decay.
1999,
Pubmed
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Katahira,
The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human.
1999,
Pubmed
Kataoka,
Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm.
2000,
Pubmed
,
Xenbase
Kim,
The Y14 protein communicates to the cytoplasm the position of exon-exon junctions.
2001,
Pubmed
,
Xenbase
Lander,
Initial sequencing and analysis of the human genome.
2001,
Pubmed
Le Hir,
Pre-mRNA splicing alters mRNP composition: evidence for stable association of proteins at exon-exon junctions.
2000,
Pubmed
Le Hir,
The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions.
2000,
Pubmed
,
Xenbase
Li,
Nonsense surveillance in lymphocytes?
1998,
Pubmed
Luo,
Splicing is required for rapid and efficient mRNA export in metazoans.
1999,
Pubmed
,
Xenbase
Lykke-Andersen,
Human Upf proteins target an mRNA for nonsense-mediated decay when bound downstream of a termination codon.
2000,
Pubmed
Lykke-Andersen,
Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1.
2001,
Pubmed
Maquat,
Mammalian heat shock p70 and histone H4 transcripts, which derive from naturally intronless genes, are immune to nonsense-mediated decay.
2001,
Pubmed
Matsumoto,
Nuclear history of a pre-mRNA determines the translational activity of cytoplasmic mRNA.
1998,
Pubmed
,
Xenbase
Mattaj,
Nucleocytoplasmic transport: the soluble phase.
1998,
Pubmed
Mayeda,
Purification and characterization of human RNPS1: a general activator of pre-mRNA splicing.
1999,
Pubmed
McGarvey,
The acute myeloid leukemia-associated protein, DEK, forms a splicing-dependent interaction with exon-product complexes.
2000,
Pubmed
Nagy,
A rule for termination-codon position within intron-containing genes: when nonsense affects RNA abundance.
1998,
Pubmed
Neu-Yilik,
Splicing and 3' end formation in the definition of nonsense-mediated decay-competent human beta-globin mRNPs.
2001,
Pubmed
Pasquinelli,
The constitutive transport element (CTE) of Mason-Pfizer monkey virus (MPMV) accesses a cellular mRNA export pathway.
1997,
Pubmed
,
Xenbase
Reed,
The role of the mammalian branchpoint sequence in pre-mRNA splicing.
1988,
Pubmed
Rodrigues,
REF proteins mediate the export of spliced and unspliced mRNAs from the nucleus.
2001,
Pubmed
,
Xenbase
Saavedra,
The simian retrovirus-1 constitutive transport element, unlike the HIV-1 RRE, uses factors required for cellular mRNA export.
1997,
Pubmed
,
Xenbase
Serin,
Identification and characterization of human orthologues to Saccharomyces cerevisiae Upf2 protein and Upf3 protein (Caenorhabditis elegans SMG-4).
2001,
Pubmed
Stutz,
REF, an evolutionary conserved family of hnRNP-like proteins, interacts with TAP/Mex67p and participates in mRNA nuclear export.
2000,
Pubmed
,
Xenbase
Thermann,
Binary specification of nonsense codons by splicing and cytoplasmic translation.
1998,
Pubmed
Vankan,
Roles of U4 and U6 snRNAs in the assembly of splicing complexes.
1992,
Pubmed
,
Xenbase
Zhang,
Intron function in the nonsense-mediated decay of beta-globin mRNA: indications that pre-mRNA splicing in the nucleus can influence mRNA translation in the cytoplasm.
1998,
Pubmed
Zhou,
The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans.
2000,
Pubmed
