Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2001 Aug 01;2015:3993-4002. doi: 10.1093/emboj/20.15.3993.
Show Gene links
Show Anatomy links
CHIF, a member of the FXYD protein family, is a regulator of Na,K-ATPase distinct from the gamma-subunit.
Béguin P
,
Crambert G
,
Guennoun S
,
Garty H
,
Horisberger JD
,
Geering K
.
???displayArticle.abstract???
The biological role of small membrane proteins of the new FXYD family is largely unknown. The best characterized FXYD protein is the gamma-subunit of the Na,K-ATPase (NKA) that modulates the Na,K-pump function in the kidney. Here, we report that, similarly to gamma(a) and gamma(b) splice variants, the FXYD protein CHIF (corticosteroid-induced factor) is a type I membrane protein which is associated with NKA in renal tissue, and modulates the Na,K-pump transport when expressed in Xenopus oocytes. In contrast to gamma(a) and gamma(b), which both decrease the apparent Na+ affinity of the Na,K-pump, CHIF significantly increases the Na+ affinity and decreases the apparent K+ affinity due to an increased Na+ competition at external binding sites. The extracytoplasmic FXYD motif is required for stable gamma-subunit and CHIF interaction with NKA, while cytoplasmic, positively charged residues are necessary for the gamma-subunit's association efficiency and for CHIF's functional effects. These data document that CHIF is a new tissue-specific regulator of NKA which probably plays a crucial role in aldosterone-responsive tissues responsible for the maintenance of body Na+ and K+ homeostasis.
Arystarkhova,
The gamma subunit modulates Na(+) and K(+) affinity of the renal Na,K-ATPase.
1999, Pubmed
Arystarkhova,
The gamma subunit modulates Na(+) and K(+) affinity of the renal Na,K-ATPase.
1999,
Pubmed
Attali,
A corticosteroid-induced gene expressing an "IsK-like" K+ channel activity in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Barlet-Bas,
Difference in the Na affinity of Na(+)-K(+)-ATPase along the rabbit nephron: modulation by K.
1990,
Pubmed
Brennan,
Acute regulation by corticosteroids of channel-inducing factor gene messenger ribonucleic acid in the distal colon.
1999,
Pubmed
,
Xenbase
Béguin,
The gamma subunit is a specific component of the Na,K-ATPase and modulates its transport function.
1997,
Pubmed
,
Xenbase
Capurro,
Cellular localization and regulation of CHIF in kidney and colon.
1996,
Pubmed
,
Xenbase
Crambert,
Transport and pharmacological properties of nine different human Na, K-ATPase isozymes.
2000,
Pubmed
,
Xenbase
Donnet,
Thermal denaturation of the Na,K-ATPase provides evidence for alpha-alpha oligomeric interaction and gamma subunit association with the C-terminal domain.
2001,
Pubmed
Forbush,
Characterization of a new photoaffinity derivative of ouabain: labeling of the large polypeptide and of a proteolipid component of the Na, K-ATPase.
1978,
Pubmed
Fu,
E2a-Pbx1 induces aberrant expression of tissue-specific and developmentally regulated genes when expressed in NIH 3T3 fibroblasts.
1997,
Pubmed
Féraille,
Sodium-potassium-adenosinetriphosphatase-dependent sodium transport in the kidney: hormonal control.
2001,
Pubmed
Geering,
Topogenic motifs in P-type ATPases.
2000,
Pubmed
Geering,
Oligomerization and maturation of Na,K-ATPase: functional interaction of the cytoplasmic NH2 terminus of the beta subunit with the alpha subunit.
1996,
Pubmed
,
Xenbase
Giebisch,
Renal potassium transport: mechanisms and regulation.
1998,
Pubmed
Hasler,
Role of beta-subunit domains in the assembly, stable expression, intracellular routing, and functional properties of Na,K-ATPase.
1998,
Pubmed
,
Xenbase
Jaisser,
Modulation of the Na,K-pump function by beta subunit isoforms.
1994,
Pubmed
,
Xenbase
Jaisser,
The nongastric H+-K+-ATPases: molecular and functional properties.
1999,
Pubmed
,
Xenbase
Jones,
Embryonic expression of the putative gamma subunit of the sodium pump is required for acquisition of fluid transport capacity during mouse blastocyst development.
1997,
Pubmed
Kuster,
A new variant of the gamma subunit of renal Na,K-ATPase. Identification by mass spectrometry, antibody binding, and expression in cultured cells.
2000,
Pubmed
Mahmmoud,
Identification of a phospholemman-like protein from shark rectal glands. Evidence for indirect regulation of Na,K-ATPase by protein kinase c via a novel member of the FXYDY family.
2000,
Pubmed
Meij,
Dominant isolated renal magnesium loss is caused by misrouting of the Na(+),K(+)-ATPase gamma-subunit.
2000,
Pubmed
Mercer,
Molecular cloning and immunological characterization of the gamma polypeptide, a small protein associated with the Na,K-ATPase.
1993,
Pubmed
Minor,
The gamma subunit of the Na,K-ATPase induces cation channel activity.
1998,
Pubmed
,
Xenbase
Moorman,
Unitary anion currents through phospholemman channel molecules.
1995,
Pubmed
,
Xenbase
Morrison,
Mat-8, a novel phospholemman-like protein expressed in human breast tumors, induces a chloride conductance in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Palmer,
Purification and complete sequence determination of the major plasma membrane substrate for cAMP-dependent protein kinase and protein kinase C in myocardium.
1991,
Pubmed
Pu,
Functional role and immunocytochemical localization of the gamma a and gamma b forms of the Na,K-ATPase gamma subunit.
2001,
Pubmed
Shi,
Membrane topology and immunolocalization of CHIF in kidney and intestine.
2001,
Pubmed
Simmerman,
Phospholamban: protein structure, mechanism of action, and role in cardiac function.
1998,
Pubmed
Suessbrich,
The IKs channel: coassembly of IsK (minK) and KvLQT1 proteins.
1999,
Pubmed
Sweadner,
The FXYD gene family of small ion transport regulators or channels: cDNA sequence, protein signature sequence, and expression.
2000,
Pubmed
Therien,
Tissue-specific distribution and modulatory role of the gamma subunit of the Na,K-ATPase.
1997,
Pubmed
Therien,
Expression and functional role of the gamma subunit of the Na, K-ATPase in mammalian cells.
1999,
Pubmed
Wald,
Differential regulation of CHIF mRNA by potassium intake and aldosterone.
1997,
Pubmed
,
Xenbase
