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In the developing vertebrate retina, progenitor cell proliferation must be precisely regulated to ensure appropriate formation of the mature tissue. Cyclin kinase inhibitors have been implicated as important regulators of proliferation during development by blocking the activity of cyclin-cyclin-dependent kinase complexes. We have found that the p27(Kip1) cyclin kinase inhibitor regulates progenitor cell proliferation throughout retinal histogenesis. p27(Kip1) is upregulated during the late G(2)/early G(1) phase of the cell cycle in retinal progenitor cells, where it interacts with the major retinal D-type cyclin-cyclin D1. Mice deficient for p27(Kip1) exhibited an increase in the proportion of mitotic cells throughout development as well as extensive apoptosis, particularly during the later stages of retinal histogenesis. Retroviral-mediated overexpression of p27(Kip1) in mitotic retinal progenitor cells led to premature cell cycle exit yet had no dramatic effects on Müller glial or bipolar cell fate specification as seen with the Xenopus cyclin kinase inhibitor, p27(Xic1). Consistent with the overexpression of p27(Kip1), mice lacking one or both alleles of p27(Kip1) maintained the same relative ratios of each major retinal cell type as their wild-type littermates. During the embryonic stages of development, when both p27(Kip1) and p57(Kip2) are expressed in retinal progenitor cells, they were found in distinct populations, demonstrating directly that different retinal progenitor cells are heterogeneous with respect to their expression of cell cycle regulators.
Alexiades,
Quantitative analysis of proliferation and cell cycle length during development of the rat retina.
1996, Pubmed
Alexiades,
Quantitative analysis of proliferation and cell cycle length during development of the rat retina.
1996,
Pubmed
Alexiades,
Subsets of retinal progenitors display temporally regulated and distinct biases in the fates of their progeny.
1997,
Pubmed
Belliveau,
Extrinsic and intrinsic factors control the genesis of amacrine and cone cells in the rat retina.
1999,
Pubmed
Belliveau,
Late retinal progenitor cells show intrinsic limitations in the production of cell types and the kinetics of opsin synthesis.
2000,
Pubmed
Bobrow,
Catalyzed reporter deposition, a novel method of signal amplification. II. Application to membrane immunoassays.
1991,
Pubmed
Cepko,
Lineage analysis using retroviral vectors.
1998,
Pubmed
Cepko,
Cell fate determination in the vertebrate retina.
1996,
Pubmed
Chiu,
Murine and bovine blue cone pigment genes: cloning and characterization of two new members of the S family of visual pigments.
1994,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Coats,
A new pathway for mitogen-dependent cdk2 regulation uncovered in p27(Kip1)-deficient cells.
1999,
Pubmed
De Leeuw,
Immunolocalization of cellular retinol-, retinaldehyde- and retinoic acid-binding proteins in rat retina during pre- and postnatal development.
1990,
Pubmed
Dyer,
The p57Kip2 cyclin kinase inhibitor is expressed by a restricted set of amacrine cells in the rodent retina.
2001,
Pubmed
Dyer,
p57(Kip2) regulates progenitor cell proliferation and amacrine interneuron development in the mouse retina.
2000,
Pubmed
Dyer,
Control of Müller glial cell proliferation and activation following retinal injury.
2000,
Pubmed
Fantl,
Mice lacking cyclin D1 are small and show defects in eye and mammary gland development.
1995,
Pubmed
Farrington,
Winged-helix, Hedgehog and Bmp genes are differentially expressed in distinct cell layers of the murine yolk sac.
1997,
Pubmed
Fero,
A syndrome of multiorgan hyperplasia with features of gigantism, tumorigenesis, and female sterility in p27(Kip1)-deficient mice.
1996,
Pubmed
Fields-Berry,
A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina.
1992,
Pubmed
Gomer,
A cell-cycle phase-associated cell-type choice mechanism monitors the cell cycle rather than using an independent timer.
1996,
Pubmed
Holt,
Cellular determination in the Xenopus retina is independent of lineage and birth date.
1988,
Pubmed
,
Xenbase
Kiyokawa,
Enhanced growth of mice lacking the cyclin-dependent kinase inhibitor function of p27(Kip1).
1996,
Pubmed
Lee,
Mice deficient for Rb are nonviable and show defects in neurogenesis and haematopoiesis.
1992,
Pubmed
Levine,
p27(Kip1) regulates cell cycle withdrawal of late multipotent progenitor cells in the mammalian retina.
2000,
Pubmed
Ma,
A unique pattern of photoreceptor degeneration in cyclin D1 mutant mice.
1998,
Pubmed
Molday,
Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes.
1983,
Pubmed
,
Xenbase
Morrow,
Two phases of rod photoreceptor differentiation during rat retinal development.
1998,
Pubmed
Nakayama,
Mice lacking p27(Kip1) display increased body size, multiple organ hyperplasia, retinal dysplasia, and pituitary tumors.
1996,
Pubmed
Ohnuma,
p27Xic1, a Cdk inhibitor, promotes the determination of glial cells in Xenopus retina.
1999,
Pubmed
,
Xenbase
Onoda,
A monoclonal antibody specific for a subpopulation of retinal bipolar cells in the frog and other vertebrates.
1987,
Pubmed
Sicinski,
Cyclin D1 provides a link between development and oncogenesis in the retina and breast.
1995,
Pubmed
Sánchez,
Transcriptional control of the cell cycle.
1996,
Pubmed
Turner,
A common progenitor for neurons and glia persists in rat retina late in development.
,
Pubmed
Turner,
Lineage-independent determination of cell type in the embryonic mouse retina.
1990,
Pubmed
Wang,
A visual pigment from chicken that resembles rhodopsin: amino acid sequence, gene structure, and functional expression.
1992,
Pubmed
Woo,
p130 and p107 use a conserved domain to inhibit cellular cyclin-dependent kinase activity.
1997,
Pubmed
Young,
Cell differentiation in the retina of the mouse.
1985,
Pubmed
Zhang,
Altered cell differentiation and proliferation in mice lacking p57KIP2 indicates a role in Beckwith-Wiedemann syndrome.
1997,
Pubmed
Zhang,
Cooperation between the Cdk inhibitors p27(KIP1) and p57(KIP2) in the control of tissue growth and development.
1998,
Pubmed
Zhu,
p107 uses a p21CIP1-related domain to bind cyclin/cdk2 and regulate interactions with E2F.
1995,
Pubmed
