Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2001 Jun 01;2011:2835-43. doi: 10.1093/emboj/20.11.2835.
Show Gene links
Show Anatomy links
Heme mediates derepression of Maf recognition element through direct binding to transcription repressor Bach1.
Ogawa K
,
Sun J
,
Taketani S
,
Nakajima O
,
Nishitani C
,
Sassa S
,
Hayashi N
,
Yamamoto M
,
Shibahara S
,
Fujita H
,
Igarashi K
.
???displayArticle.abstract???
Heme controls expression of genes involved in the synthesis of globins and heme. The mammalian transcription factor Bach1 functions as a repressor of the Maf recognition element (MARE) by forming antagonizing hetero-oligomers with the small Maf family proteins. We show here that heme binds specifically to Bach1 and regulates its DNA-binding activity. Deletion studies demonstrated that a heme-binding region of Bach1 is confined within its C-terminal region that possesses four dipeptide cysteine-proline (CP) motifs. Mutations in all of the CP motifs of Bach1 abolished its interaction with heme. The DNA-binding activity of Bach1 as a MafK hetero-oligomer was markedly inhibited by heme in gel mobility shift assays. The repressor activity of Bach1 was lost upon addition of hemin in transfected cells. These results suggest that increased levels of heme inactivate the repressor Bach1, resulting in induction of a host of genes with MARES:
Alam,
Nrf2, a Cap'n'Collar transcription factor, regulates induction of the heme oxygenase-1 gene.
1999, Pubmed
Alam,
Nrf2, a Cap'n'Collar transcription factor, regulates induction of the heme oxygenase-1 gene.
1999,
Pubmed
Blouin,
Isolation of the human BACH1 transcription regulator gene, which maps to chromosome 21q22.1.
1998,
Pubmed
Chen,
Amino acid microsequencing of internal tryptic peptides of heme-regulated eukaryotic initiation factor 2 alpha subunit kinase: homology to protein kinases.
1991,
Pubmed
Chen,
Cloning of the cDNA of the heme-regulated eukaryotic initiation factor 2 alpha (eIF-2 alpha) kinase of rabbit reticulocytes: homology to yeast GCN2 protein kinase and human double-stranded-RNA-dependent eIF-2 alpha kinase.
1991,
Pubmed
Creusot,
CYP1 (HAP1) regulator of oxygen-dependent gene expression in yeast. I. Overall organization of the protein sequence displays several novel structural domains.
1988,
Pubmed
Crosby,
Regulation of hemoglobin synthesis and proliferation of differentiating erythroid cells by heme-regulated eIF-2alpha kinase.
2000,
Pubmed
Fujita,
Erythroleukemia differentiation. Distinctive responses of the erythroid-specific and the nonspecific delta-aminolevulinate synthase mRNA.
1991,
Pubmed
Fukuda,
Regulation of beta-globin mRNA accumulation by heme in dimethyl sulfoxide (DMSO)-sensitive and DMSO-resistant murine erythroleukemia cells.
1994,
Pubmed
Fytlovich,
Evidence for an interaction between the CYP1(HAP1) activator and a cellular factor during heme-dependent transcriptional regulation in the yeast Saccharomyces cerevisiae.
1993,
Pubmed
Harigae,
Deficient heme and globin synthesis in embryonic stem cells lacking the erythroid-specific delta-aminolevulinate synthase gene.
1998,
Pubmed
Hon,
Functional analysis of heme regulatory elements of the transcriptional activator Hap1.
2000,
Pubmed
Igarashi,
Regulation of transcription by dimerization of erythroid factor NF-E2 p45 with small Maf proteins.
1994,
Pubmed
Igarashi,
Activity and expression of murine small Maf family protein MafK.
1995,
Pubmed
Igarashi,
Multivalent DNA binding complex generated by small Maf and Bach1 as a possible biochemical basis for beta-globin locus control region complex.
1998,
Pubmed
Inamdar,
The heme-responsive element of the mouse heme oxygenase-1 gene is an extended AP-1 binding site that resembles the recognition sequences for MAF and NF-E2 transcription factors.
1996,
Pubmed
Ishii,
Transcription factor Nrf2 coordinately regulates a group of oxidative stress-inducible genes in macrophages.
2000,
Pubmed
Johnsen,
Interaction of the CNC-bZIP factor TCF11/LCR-F1/Nrf1 with MafG: binding-site selection and regulation of transcription.
1998,
Pubmed
Kataoka,
Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.
1995,
Pubmed
Kobayashi,
Molecular cloning and functional characterization of a new Cap'n' collar family transcription factor Nrf3.
1999,
Pubmed
Kobayashi,
A combinatorial code for gene expression generated by transcription factor Bach2 and MAZR (MAZ-related factor) through the BTB/POZ domain.
2000,
Pubmed
Kotkow,
Dependence of globin gene expression in mouse erythroleukemia cells on the NF-E2 heterodimer.
1995,
Pubmed
Kramer,
Transcriptional regulation of the murine erythroid-specific 5-aminolevulinate synthase gene.
2000,
Pubmed
Lathrop,
Regulation by heme of mitochondrial protein transport through a conserved amino acid motif.
1993,
Pubmed
Marini,
hMAF, a small human transcription factor that heterodimerizes specifically with Nrf1 and Nrf2.
1997,
Pubmed
McCoubrey,
Heme oxygenase-2 is a hemoprotein and binds heme through heme regulatory motifs that are not involved in heme catalysis.
1997,
Pubmed
Monson,
The FixL protein of Rhizobium meliloti can be separated into a heme-binding oxygen-sensing domain and a functional C-terminal kinase domain.
1992,
Pubmed
Motohashi,
The world according to Maf.
1997,
Pubmed
Muto,
Identification of Bach2 as a B-cell-specific partner for small maf proteins that negatively regulate the immunoglobulin heavy chain gene 3' enhancer.
1998,
Pubmed
Ohira,
Characterization of a human homolog (BACH1) of the mouse Bach1 gene encoding a BTB-basic leucine zipper transcription factor and its mapping to chromosome 21q22.1.
1998,
Pubmed
Oyake,
Bach proteins belong to a novel family of BTB-basic leucine zipper transcription factors that interact with MafK and regulate transcription through the NF-E2 site.
1996,
Pubmed
Pfeifer,
Functional dissection and sequence of yeast HAP1 activator.
1989,
Pubmed
Rafie-Kolpin,
Two heme-binding domains of heme-regulated eukaryotic initiation factor-2alpha kinase. N terminus and kinase insertion.
2000,
Pubmed
Rotenberg,
Characterization of a cDNA-encoding rabbit brain heme oxygenase-2 and identification of a conserved domain among mammalian heme oxygenase isozymes: possible heme-binding site?
1991,
Pubmed
Sassa,
The role of heme in gene expression.
1996,
Pubmed
Shibahara,
Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation.
1993,
Pubmed
Steiner,
Heme binding to a conserved Cys-Pro-Val motif is crucial for the catalytic function of mitochondrial heme lyases.
1996,
Pubmed
Taketani,
Non-enzymatic heme formation in the presence of fatty acids and thiol reductants.
1984,
Pubmed
Talbot,
The 5'HS2 of the globin locus control region enhances transcription through the interaction of a multimeric complex binding at two functionally distinct NF-E2 binding sites.
1991,
Pubmed
Toki,
Human small Maf proteins form heterodimers with CNC family transcription factors and recognize the NF-E2 motif.
1997,
Pubmed
Yamamoto,
Structure, turnover, and heme-mediated suppression of the level of mRNA encoding rat liver delta-aminolevulinate synthase.
1988,
Pubmed
Yoshida,
Long range interaction of cis-DNA elements mediated by architectural transcription factor Bach1.
1999,
Pubmed
Yoshida,
Human heme oxygenase cDNA and induction of its mRNA by hemin.
1988,
Pubmed
Zhang,
Heme binds to a short sequence that serves a regulatory function in diverse proteins.
1995,
Pubmed
Zhang,
Molecular mechanism governing heme signaling in yeast: a higher-order complex mediates heme regulation of the transcriptional activator HAP1.
1998,
Pubmed