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XB-ART-8964
Fertil Steril 2001 Jun 01;756:1194-7. doi: 10.1016/s0015-0282(01)01778-2.
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Rescue of mouse embryos from 2-cell blocks by microinjection of maturation promoting factor.

Nakano H , Kubo H .


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OBJECTIVE: To examine the rescue of mouse embryos from 2-cell blocks by the microinjection of maturation promoting factor (MPF) extracted from matured Xenopus eggs into one of the blastomeres of 2-cell stage mouse embryos. DESIGN: Controlled laboratory study. SETTING: First Department of Obstetrics and Gynecology, Toho University School of Medicine, Tokyo, Japan. ANIMAL(S): Eight- to 10-week-old female Crj:CD-1(ICR) mice. INTERVENTION(S): One of the blastomeres of the mouse 2-cell embryos was injected with MPF (MI group) or mHTF medium (MED group) at 28--32 hours after insemination. MAIN OUTCOME MEASURE(S): The developmental rate to blastocyst. RESULT(S): The developmental rate to blastocyst in the MI group (48.0%) was significantly higher than that in the MED group (0%). CONCLUSION(S): The 2-cell block was specifically rescued by the microinjection of MPF and not by the insertion of pipettes.

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