Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Plant Mol Biol
2001 Jan 01;451:51-62. doi: 10.1023/a:1006428007826.
Show Gene links
Show Anatomy links
The brassica MIP-MOD gene encodes a functional water channel that is expressed in the stigma epidermis.
Dixit R
,
Rizzo C
,
Nasrallah M
,
Nasrallah J
.
???displayArticle.abstract???
In crucifers, the ability of the stigma to differentially modulate hydration of pollen grains, depending on whether the pollen is recognized to be compatible or incompatible, represents a crucial stage in pollination. Our recent analysis of the mod mutation of Brassica, which results in a breakdown of the self-incompatibility response, led to the isolation of a gene linked to the MOD locus which is expressed at low levels in mod mutants. The gene is predicted to encode a plasma membrane-localized aquaporin-like protein and has been designated MIP-MOD. We utilized reporter gene analysis to demonstrate that the MIP-MOD promoter is active in Brassica papillar cells as well as in some vegetative tissues. The encoded protein is also likely to be plasma membrane-localized based on the observation that all plasma membrane-intrinsic aquaporin-like proteins in Brassica leaves are enriched in plasma membrane fractions. The MIP-MOD protein results in a low but measurable enhancement in osmotic water permeability of Xenopus oocytes and hence represents a functional aquaporin. The results are consistent with the notion that MIP-MOD is involved in the regulation of water transport across the stigma epidermal cell membrane.
Barrieu,
High expression of the tonoplast aquaporin ZmTIP1 in epidermal and conducting tissues of maize.
1998, Pubmed
Barrieu,
High expression of the tonoplast aquaporin ZmTIP1 in epidermal and conducting tissues of maize.
1998,
Pubmed
Barrieu,
Tonoplast intrinsic proteins from cauliflower (Brassica oleracea L. var. botrytis): immunological analysis, cDNA cloning and evidence for expression in meristematic tissues.
1998,
Pubmed
Biela,
The Nicotiana tabacum plasma membrane aquaporin NtAQP1 is mercury-insensitive and permeable for glycerol.
1999,
Pubmed
,
Xenbase
Borgnia,
Cellular and molecular biology of the aquaporin water channels.
1999,
Pubmed
Brimecombe,
Functional consequences of NR2 subunit composition in single recombinant N-methyl-D-aspartate receptors.
1997,
Pubmed
Chaumont,
Plasma membrane intrinsic proteins from maize cluster in two sequence subgroups with differential aquaporin activity.
2000,
Pubmed
,
Xenbase
Chaumont,
Characterization of a maize tonoplast aquaporin expressed in zones of cell division and elongation.
1998,
Pubmed
,
Xenbase
Chrispeels,
Aquaporins: the molecular basis of facilitated water movement through living plant cells?
1994,
Pubmed
Daniels,
Characterization of a new vacuolar membrane aquaporin sensitive to mercury at a unique site.
1996,
Pubmed
Daniels,
The plasma membrane of Arabidopsis thaliana contains a mercury-insensitive aquaporin that is a homolog of the tonoplast water channel protein TIP.
1994,
Pubmed
,
Xenbase
Dean,
Purification and functional reconstitution of soybean nodulin 26. An aquaporin with water and glycerol transport properties.
1999,
Pubmed
,
Xenbase
Fry,
Transformation of Brassica napus with Agrobacterium tumefaciens based vectors.
1987,
Pubmed
Fyfe,
Subunit composition determines the single channel kinetics of the epithelial sodium channel.
1998,
Pubmed
,
Xenbase
Gerbeau,
Aquaporin Nt-TIPa can account for the high permeability of tobacco cell vacuolar membrane to small neutral solutes.
1999,
Pubmed
,
Xenbase
Goring,
The S-locus receptor kinase gene in a self-incompatible Brassica napus line encodes a functional serine/threonine kinase.
1992,
Pubmed
Hasegawa,
Molecular cloning of a mercurial-insensitive water channel expressed in selected water-transporting tissues.
1994,
Pubmed
,
Xenbase
Hatt,
Modification of glutamate receptor channels: molecular mechanisms and functional consequences.
1999,
Pubmed
Hülskamp,
Identification of genes required for pollen-stigma recognition in Arabidopsis thaliana.
1995,
Pubmed
Ikeda,
An aquaporin-like gene required for the Brassica self-incompatibility response.
1997,
Pubmed
Johansson,
Water transport activity of the plasma membrane aquaporin PM28A is regulated by phosphorylation.
1998,
Pubmed
,
Xenbase
Kaldenhoff,
Significance of plasmalemma aquaporins for water-transport in Arabidopsis thaliana.
1998,
Pubmed
,
Xenbase
Kammerloher,
Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system.
1994,
Pubmed
,
Xenbase
Katavic,
In planta transformation of Arabidopsis thaliana.
1994,
Pubmed
Ma,
Cloning of a water channel homolog expressed in brain meningeal cells and kidney collecting duct that functions as a stilbene-sensitive glycerol transporter.
1994,
Pubmed
,
Xenbase
Ma,
Defective secretion of saliva in transgenic mice lacking aquaporin-5 water channels.
1999,
Pubmed
Maric,
GABAA receptor subunit composition and functional properties of Cl- channels with differential sensitivity to zolpidem in embryonic rat hippocampal cells.
1999,
Pubmed
Maurel,
Phosphorylation regulates the water channel activity of the seed-specific aquaporin alpha-TIP.
1995,
Pubmed
,
Xenbase
Maurel,
Purified vesicles of tobacco cell vacuolar and plasma membranes exhibit dramatically different water permeability and water channel activity.
1997,
Pubmed
Nasrallah,
Signaling the arrest of pollen tube development in self-incompatible plants.
1994,
Pubmed
Niemietz,
Characterization of Water Channels in Wheat Root Membrane Vesicles.
1997,
Pubmed
Park,
Phylogenetic characterization of the MIP family of transmembrane channel proteins.
1996,
Pubmed
Preston,
Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein.
1992,
Pubmed
,
Xenbase
Preston,
The mercury-sensitive residue at cysteine 189 in the CHIP28 water channel.
1993,
Pubmed
,
Xenbase
Preuss,
A conditional sterile mutation eliminates surface components from Arabidopsis pollen and disrupts cell signaling during fertilization.
1993,
Pubmed
Schnermann,
Defective proximal tubular fluid reabsorption in transgenic aquaporin-1 null mice.
1998,
Pubmed
Sharp,
Growth of the maize primary root at low water potentials : I. Spatial distribution of expansive growth.
1988,
Pubmed
Stein,
A plant receptor-like gene, the S-locus receptor kinase of Brassica oleracea L., encodes a functional serine/threonine kinase.
1993,
Pubmed
Stein,
SRK, the stigma-specific S locus receptor kinase of Brassica, is targeted to the plasma membrane in transgenic tobacco.
1996,
Pubmed
Toriyama,
Transformation of Brassica oleracea with an S-locus gene from B. campestris changes the self-incompatibility phenotype.
1991,
Pubmed
Varadaraj,
The role of MIP in lens fiber cell membrane transport.
1999,
Pubmed
Wall,
Isolation of plasma membrane complexes from Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Ward,
Dissociation and Reassembly of the Vacuolar H-ATPase Complex from Oat Roots.
1992,
Pubmed
Yamada,
A family of transcripts encoding water channel proteins: tissue-specific expression in the common ice plant.
1995,
Pubmed
,
Xenbase
Yang,
Water and glycerol permeabilities of aquaporins 1-5 and MIP determined quantitatively by expression of epitope-tagged constructs in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Zhang,
Water and urea permeability properties of Xenopus oocytes: expression of mRNA from toad urinary bladder.
1991,
Pubmed
,
Xenbase