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XB-ART-9635
Mech Dev February 1, 2001; 100 (2): 275-89.
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Functional characterization and genetic mapping of alk8.

Payne TL , Postlethwait JH , Yelick PC .


Abstract
The novel type I TGFbeta family member receptor alk8 is expressed both maternally and zygotically. Functional characterization of alk8 was performed using microinjection studies of constitutively active (CA), kinase modified/dominant negative (DN), and truncated alk8 mRNAs. CA Alk8 expression produces ventralized embryos while DN Alk8 expression results in dorsalized phenotypes. Truncated alk8 expressing embryos display a subtle dorsalized phenotype closely resembling that of the identified zebrafish dorsalized mutant, lost-a-fin (laf). Single-strand conformation polymorphism (SSCP) analysis was used to map alk8 to zebrafish LG02 in a region demonstrating significant conserved synteny to Hsa2, and which contains the human alk2 gene, ACVRI. Altogether, these functional, gene mapping and phylogenetic analyses suggest that alk8 may be the zebrafish orthologue to human ACVRI (alk2), and therefore extend previous studies of Alk2 conducted in Xenopus.

PubMed ID: 11165484
Article link: Mech Dev
Grant support: [+]

Species referenced: Xenopus
Genes referenced: acvr1 bmp4 chrd.1 egr2 myod1 otx2 tgfb1


Article Images: [+] show captions