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Dev Biol February 15, 2001; 230 (2): 217-29.
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Overexpression of the Xenopus tight-junction protein claudin causes randomization of the left-right body axis.

Brizuela BJ , Wessely O , De Robertis EM .

This study presents Xenopus claudin (Xcla), a tight-junction protein that is abundantly expressed in eggs and neuroectodermal precursors during early development. It was isolated via a differential screen for mRNAs enriched in microsomes in the Xenopus blastula. The Xcla protein contains four transmembrane domains and a carboxy-terminal cytoplasmic region with a putative PDZ-binding site. We show that this PDZ-binding site of Xcla is critical for its correct localization on the cell membrane and that a truncated form leads to delocalization of the tight-junction protein ZO-1. Overexpression of Xcla causes changes in the cell adhesion properties of blastomeres and leads to visceral situs randomization. The results suggest that left-right axial patterning is very sensitive to changes in regulation of cell-cell interactions and implicate a tight-junction protein in the determination of left-right asymmetry.

PubMed ID: 11161574
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus
Genes referenced: actl6a cldn6.2 gdf1 h4c4 nodal1 ocln prl.1 tjp1 tjp2 tjp3

Article Images: [+] show captions