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XB-ANTIBODY-16325890

Antibody Name: hpse2 Ab1

Common Name: heparanase 2

RRID:
Synonyms:

Antigens
Gene: hpse2

Clone Type: unknown
Source:

Antibody
Clone Number
Purification
Isotype isotype unknown
Host Organism rabbit
Xenopus Reactivity tropicalis
Non-Xenopus Reactivity
Description:
Immunogen
Name
Type polypeptide
Post Translational Modifications None
Source Organism frog
Description A custom rabbit anti-heparanase 2 antibody was designed and manufactured (Generon). The immunising peptide sequence used was QLDPSIIHDGWLDC (Figure 1)
Sequence [+]
Reported Usage
western blot To test specificity, anti-heparanase 2 antibody was incubated overnight at 4°C on a rotator with a 50 fold excess immunising peptide or a control reaction of 50 fold excess non-specific peptide, prior to use in immunohistochemistry applications. Western blotting was performed by standard methods. 22  Downloaded from http://hmg.oxfordjournals.org/ at Cincinnati Children's Hospital Library on April 8, 2014 Total protein was extracted from pools of three embryos, with amounts equivalent to one embryo separated via SDS-PAGE.
Publications
First: Heparanase 2, mutated in urofacial syndrome, mediates peripheral neural development in Xenopus., Hum Mol Genet 2014                                
Most recent:
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