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XB-ANTIBODY-14579146

Antibody Name: Itgb1 Ab1

Common Name: integrin beta-1

RRID: AB_528309
Synonyms: integrin beta-1, 8C8, beta-1 integrin

Antigens
Gene: itgb1

Clone Type: Monoclonal
Source: Developmental Studies Hybridoma Bank

Antibody
Clone Number 8C8
Purification
Isotype IgG1
Host Organism mouse
Xenopus Reactivity tropicalis, laevis
Non-Xenopus Reactivity zebrafish
Description: Supplied by DSHB, Cat# 8C8 Citation: (DSHB Cat# 8c8, RRID:AB_528309). Please email citation to DSHB.
Immunogen
Name integrin beta-1
Type polypeptide
Post Translational Modifications None
Source Organism frog
Description protein fraction enriched in beta1-integrin precursor protein Predicted: 86 kDa; Apparent: 100 kDa (precursor) and 115 kDa (mature protein) under non-reducing conditions and 125 kDa (precursor) and 135 kDa (mature protein) under reducing conditions 8C8 doesn't recognize the reduced protein. For immunohistochemistry, formaldehyde or methanol fixation work well.
Sequence
Reported Usage
western blot must be in the unreduced state; antibody does not recognize reduced protein
immunoprecipitation
immunofluorescence MEMFA fix 1-2 hrs @ RT, post-fix in Dents. Use at 1:300
Publications
First: Beta 1-integrin is a maternal protein that is inserted into all newly formed plasma membranes during early Xenopus embryogenesis., Development 1992  
Most recent: Molecular markers for corneal epithelial cells in larval vs. adult Xenopus frogs., Exp Eye Res 2019                          
View All Papers