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Summary Images Attributions Wiki Source
XB-ANTIBODY-14579391

Antibody Name: En2 Ab1

Common Name: Engrailed

RRID: AB_528224
Synonyms: 4D9, 4D9 anti-engrailed/invected, 4D9.D4, Invected

Antigens
Gene: en2 , en1
XAO: midbrain-hindbrain boundary

Clone Type: Monoclonal
Source: Developmental Studies Hybridoma Bank

Antibody
Clone Number 4D9
Purification Affinity Purification
Isotype IgG1
Host Organism mouse
Xenopus Reactivity tropicalis, laevis
Non-Xenopus Reactivity chicken, fruitfly, worm, zebrafish
Description: Supplied by DSHB, Cat# 4D9, Cite this: (DSHB Cat# 4D9 anti-engrailed/invected, RRID:AB_528224),. This MAB anti-engrailed/invected, is available from EXRC as 4D9.D4 Please email the citation to DSHB.
Immunogen
Name engrailed-Invected
Type polypeptide
Post Translational Modifications None
Source Organism Other
Description C-terminal two-thirds of the invected protein of Drosophila melanogaster. This antibody recognizes both the engrailed and invected gene products of Drosophila and the epitope has been localized to residues 38-58 of the homeodomain. aa 38-48 of the engrailed homeobox (C-terminal aa 490-500 of engrailed: ELGLNEAQIKI; aa 507-517 of invected)
Sequence
Reported Usage
immunohistochemistry
western blot
immunoprecipitation
immunofluorescence
chromatin immunoprecipitation
Publications
First: Expression of an engrailed-related protein is induced in the anterior neural ectoderm of early Xenopus embryos., Development 1989                    
Most recent: The microtubule plus end tracking protein Orbit/MAST/CLASP acts downstream of the tyrosine kinase Abl in mediating axon guidance., Neuron 2004  
View All Papers