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Summary Images Attributions Wiki Source
XB-ANTIBODY-22041796

Antibody Name: Rdd Ab1

Common Name: anti-rdd

RRID:
Synonyms: Rdd3, Rdd4

Antigens
Gene: rdd4 , rdd3

Clone Type: Polyclonal
Source:

Antibody
Clone Number
Purification Affinity Purification
Isotype IgG
Host Organism rabbit
Xenopus Reactivity X. laevis
Non-Xenopus Reactivity
Description: Custom Antibody against peptide common to Rdd3 and Rdd4 developed by Mitsugu lab.
Immunogen
Name rdd
Type synthetic sequence
Post Translational Modifications None
Source Organism frog
Description A common amino acid sequence in Rdd3 and Rdd4, SQCPPSQEQRCPLNQFWE (amino acids 149-166 of Rdd3, amino acids 214-231 of Rdd4), was selected as the antigenic and hydrophilic site to produce anti-Rdd peptide serum. Rdd peptide was synthesized and conjugated with keyhole limpet hemocyanin.
Sequence [+]
Reported Usage
immunohistochemistry To detect endogenous Rdd proteins, albino embryos were incubated with anti-rdd antibody as a first antibody and were incubated with AP-conjugated anti-rabbit IgG as a second antibody. The reaction was visualized in NBT/BCIP solution. Rdd pep tides (20 µg/ml) were added with anti-Rdd serum for an antibody absorption experiment.
Publications
???displayAntibody.papersFirst??? Expression and localization of Rdd proteins in Xenopus embryo., Anat Cell Biol 2014            
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