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Summary Attributions Wiki
XB-GRNA-23659092
gRNA Name: adprhl1 gRNA6
Synonyms: -e1-1
Genomic Target: adprhl1

PAM Sequence: TGG
Sequence: 5' GGCAAGAAGAGCTAAAACAACT 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/22 20/22 20/22
genome
gRNA
 
5' AGTTGTTTTAGCTCTTCTTGGA 3'
   ||||||||||||||||||||  
3' TCAACAAAATCGAGAAGAACGG 5'
 
5' AGTTGTTTTAGCTCTTCTTGGA 3'
   ||||||||||||||||||||  
3' TCAACAAAATCGAGAAGAACGG 5'
 
5' AGTTGTTTTAGCTCTTCTTGGA 3'
   ||||||||||||||||||||  
3' TCAACAAAATCGAGAAGAACGG 5'
    
Genomic Alignments Position Identity Strand Genomic Target
laevis 10.1 Chr2L:120467490..120467511 20/22 Sense target adprhl1.L
Chr2S:106623238..106623259 20/22 Sense target adprhl1.S
Chr2L:60277546..60277567 20/22 Sense off-target asap3.L
Chr2S:96711405..96711426 19/22 Antisense off-target impg2.S
Chr4L:109552790..109552811 19/22 Antisense off-target kcnt2.L
Chr2L:147377892..147377913 19/22 Antisense off-target r3hdm2.L
Chr2L:70850637..70850658 19/22 Antisense off-target LOC108708146
Chr2L:70850895..70850916 19/22 Antisense off-target LOC108708146
Chr1S:195755017..195755038 18/22 Antisense off-target syt4.S
Chr4S:12300838..12300859 18/22 Sense off-target madd.S
Chr7L:92121841..92121862 18/22 Antisense off-target h6pd.L
Chr8L:86700562..86700583 20/22 unknown
Chr3L:92410753..92410774 19/22 unknown
Chr1S:7329401..7329422 18/22 unknown
Chr2L:154311019..154311040 18/22 unknown
Chr3L:22451986..22452007 18/22 unknown
Chr3S:11989123..11989144 18/22 unknown
Chr4S:94364183..94364204 18/22 unknown
Chr5L:134411494..134411515 18/22 unknown
Chr5S:112953938..112953959 18/22 unknown
Chr6L:40240688..40240709 18/22 unknown
tropicalis 10.0 Chr2:115893203..115893224 20/22 Sense adprhl1
Chr4:86412371..86412392 19/22 Sense hook1
Chr5:30027431..30027452 19/22 Sense pcsk2
Chr1:68935294..68935315 18/22 Sense zgrf1
Chr3:33706006..33706027 19/22
Chr7:43284486..43284507 19/22
Chr8:85716437..85716458 19/22
Chr5:125255019..125255040 18/22
Publications
???morpholino.displaySummary.papersFirst??? Defective heart chamber growth and myofibrillogenesis after knockout of adprhl1 gene function by targeted disruption of the ancestral catalytic active site., PLoS One 2020                                              
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