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Summary Attributions Wiki
XB-GRNA-23659097
gRNA Name: adprhl1 gRNA11
Synonyms: -e7-2S
Genomic Target: adprhl1

PAM Sequence: TGG
Sequence: 5' GGTGTCTTTATGGATTGCTCTA 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 22/22 21/22 22/22
genome
gRNA
 
5' TAGAGCAATCCATAAAGACACC 3'
   ||||||||||||||||||||||
3' ATCTCGTTAGGTATTTCTGTGG 5'
 
5' TAGAGCAATCCATACAGACACC 3'
   |||||||||||||| |||||||
3' ATCTCGTTAGGTATTTCTGTGG 5'
 
5' TAGAGCAATCCATAAAGACACC 3'
   ||||||||||||||||||||||
3' ATCTCGTTAGGTATTTCTGTGG 5'
    
Genomic Alignments Position Identity Strand Genomic Target
laevis 10.1 Chr2S:106633829..106633850 22/22 Sense target adprhl1.S
Chr2L:120477625..120477646 21/22 Sense target adprhl1.L
Chr4L:138194125..138194146 19/22 Sense off-target lamb2.L
Chr6L:5039776..5039797 19/22 Sense off-target rnf121.L
Chr3L:101440388..101440409 18/22 Sense off-target shf.L
Chr1S:90318951..90318972 18/22 Antisense off-target efna2.S
Chr6L:86714840..86714861 18/22 Sense off-target LOC108718521
Chr2L:48665556..48665577 19/22 unknown
Chr3S:54034881..54034902 19/22 unknown
Chr4S:53705491..53705512 18/22 unknown
tropicalis 10.0 Chr2:115908706..115908727 22/22 Sense adprhl1
Chr3:100147555..100147576 19/22
Chr4:54584431..54584452 19/22
Publications
???morpholino.displaySummary.papersFirst??? Defective heart chamber growth and myofibrillogenesis after knockout of adprhl1 gene function by targeted disruption of the ancestral catalytic active site., PLoS One 2020                                              
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